Versatile vector tools for efficient protein screening across multiple expression systems
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Farming Systems, Food Security, Dietary Intakes, and Nutrition Status Among Young Children in Rural Tanzania Before and After Harvest. [PDF]
Muhimbula H, Kinabo J, O'Sullivan AM.
europepmc +1 more source
Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta +7 more
wiley +1 more source
Influence of Shading on Essential Oil Quantity and Quality of Sage (<i>Salvia officinalis</i> L.) at Different Harvest Times. [PDF]
Milenković L +9 more
europepmc +1 more source
The crystal structure of Borrelia burgdorferi nicotinamidase (PncA/BBE22) reveals the correct full‐length protein initiated from a non‐canonical AUU start codon. The structure validates previous biochemical findings and resolves a long‐standing annotation error, demonstrating that the truncated database sequence is structurally incompatible with the ...
Kalvis Brangulis
wiley +1 more source
Fast Phenotypic and Genetic Changes in Life History and Mortality of Experimental Populations of Guppies (<i>Poecilia reticulata</i>) Exposed to Size-Dependent Harvest in a Decade-Long Selection Experiment. [PDF]
Diaz Pauli B +6 more
europepmc +1 more source
Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard +12 more
wiley +1 more source
Influence of cultivar, fruit maturity, and harvest month on the targeted metabolite profile of Highland strawberries (<i>Fragaria</i> × <i>ananassa</i> Duch.). [PDF]
Kim JY +8 more
europepmc +1 more source

