Results 111 to 120 of about 3,678,063 (310)

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Performance Analysis and Rapid Optimization of Vehicle ORC Systems Based on Numerical Simulation and Machine Learning

Energies
The organic Rankine cycle (ORC) system is an important technology for recovering energy from the waste heat of internal combustion engines, which is of significant importance for the improvement of fuel utilization. This study analyses the performance of
Xin Wang, Xia Chen, Chengda Xing, Xu Ping, Hongguang Zhang, Fubin Yang   +5 more
doaj   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Transition from film boiling to nucleate boiling in forced convection vertical flow [PDF]

, 1972
The mechanism of collapse of forced cnnvection annular vertical flow film boiling, with liquid core, is investigated using liquid nitrogen at low pressures. The report includes the effect of heat flux from the buss bar.
Iloeje, Onwuamaeze C., Plummer, David N., Rohsenow, Warren M.   +2 more
core  

Boiling and Evaporation in Small Diameter Channels

, 2003
Since the 1950s, the research and industrial communities have developed a body of experimental data and set of analytical tools and correlations for two-phase flow and heat transfer in passages having a hydraulic diameter greater than about 6 mm.
Arthur E. Bergles, Bergles, Arthur E., Peter Griffith, Lienhard, John H, John H. Lienhard V, Kendall, Gail E., Griffith, Peter, Gail E. Kendall   +7 more
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Oil Circulation Effects on Evaporation Heat Transfer in Brazed Plate Heat Exchanger using R134A [PDF]

, 2012
Experimental study was performed for oil circulation effects on evaporation heat transfer in the brazed type plate heat exchangers using R134A. In this study, distribution device was installed to ensure uniform flow distribution in the refrigerant flow ...
Chang, Youngsoo, Jang, Jaekyoo, Kang, Byungha   +2 more
core   +1 more source

Modelling Of Heat Transfer In Buildings

ECMS 2011 Proceedings edited by: T. Burczynski, J. Kolodziej, A. Byrski, M. Carvalho, 2011
The main idea of this article is taking advantage of heat energy accumulation in building walls in order to design more effective heating system. Essential article goal is to simulate thermal construction responses in dependence on changing boundary conditions.
openaire   +3 more sources

Salmonella enterica serovar typhi limits the potency of typhoid toxin and ADP‐ribosylating toxin AB to establish a persistent infection

FEBS Open Bio, EarlyView.
The two catalytic subunits of typhoid toxin dissociate from the holotoxin in the ER of an intoxicated cell, but only CdtB exits the ER to generate immunosuppressive effects. PltA is retained in the ER and sequestered from its cytosolic target, thus allowing the anti‐inflammatory effects of CdtB to promote intestinal colonization.
Maria C. Zabala‐Rodriguez, Juliette K. Tinker, Teresa Frisan, Taj Azarian, Ken Teter   +4 more
wiley   +1 more source