Results 221 to 230 of about 3,101,587 (287)

Knocking Out Two Polyphenol Oxidase Genes Significantly Improves Recombinant Protein Purification in Nicotiana benthamiana

Plant Biotechnology Journal, EarlyView.
ABSTRACT Efficient purification remains a key technical challenge affecting the recovery efficiency of recombinant proteins in plant‐based systems. Complex metabolites, particularly polyphenols, often cause recombinant protein aggregation during purification.
Hai‐Ping Diao, Hui‐Xin Meng, Xue‐Jiao Xu, Zeng‐Lin Zhang, Jian‐Feng Zhang, Yong‐Feng Guo, Inhwan Hwang, Shi‐Jian Song   +7 more
wiley   +1 more source

Impacts of revised smoke-free regulations under the 2020 Japan Health Promotion Act on cigarette smoking and heated tobacco product use in indoor public places and homes: findings from 2018 to 2021 International Tobacco Control (ITC) Japan Surveys. [PDF]

Tob Control
Togawa K, Fong GT, Quah ACK, Meng G, Xu SS, Quimet J, Mochizuki Y, Yoshimi I, Odani S, Tabuchi T, Katanoda K.   +10 more
europepmc   +1 more source

Gene Editing of Nicotiana benthamiana Architecture for Space‐Efficient Production of Recombinant Proteins in Closed Environments

Plant Biotechnology Journal, EarlyView.
ABSTRACT Indoor vertical farming (VF) offers practical advantages for the cultivation of plant protein bio‐factories including plant uniformity, product consistency, water/nutrient recycling and production cycles on a year‐round basis. Much progress has been achieved toward the development of innovative systems for artificial lighting, automated ...
Béatrice Giroux, Kristina LeBreux, Louis Feyzeau, Marie‐Claire Goulet, Charles Goulet, Dominique Michaud   +5 more
wiley   +1 more source

Volumetric choice experiment to estimate the impact of e-cigarette and heated tobacco product characteristics on substitution and complementary use among adults who smoke cigarettes and recently initiated e-cigarette use. [PDF]

BMJ Open
Do VV, Shang C, Huang J, Islam T, Pechacek TF, Weaver SR.   +5 more
europepmc   +1 more source

ERF Transcription Factor NaERFIDOG Regulates JA‐Induced Defence and Growth Inhibition in Nicotiana attenuata Upon Alternaria alternata Infection

Plant Biotechnology Journal, EarlyView.
ABSTRACT Jasmonate (JA) regulates defence responses in plants. However, JA‐induced defence often results in growth inhibition. This process is largely mediated by MYC2, the master regulator of JA signalling. Nevertheless, the underlying molecular mechanisms have not yet been fully uncovered.
Luyan Zhao, Na Song, Lei Wang, Jinsong Wu   +3 more
wiley   +1 more source

Association between heated tobacco product use and worsening asthma symptoms: findings from a nationwide internet survey in Japan, 2023. [PDF]

Environ Health Prev Med
Noguchi S, Ishimaru T, Yatera K, Fujino Y, Tabuchi T.   +4 more
europepmc   +1 more source

Plant‐Based Production of Human Major Histocompatibility Complex Class II Molecules

Plant Biotechnology Journal, EarlyView.
ABSTRACT Major histocompatibility complex class II (MHC II) protein triggers adaptive immune responses by presenting antigens on antigen‐presenting cells (APCs) to be recognised by CD4+ T cells. Recombinant MHC II proteins are effective for the analysis of peptide‐binding interaction, detection of antigen‐specific T‐cells and clinical testing for donor‐
Abdelaziz Ramadan, Hiroko Miyadera, Kaho Oka, Kohei Ishikawa, Toshiya Kobayashi, Misaki Kobayashi, Mitsunori Shiroishi, Emiko Noguchi, Kenji Miura   +8 more
wiley   +1 more source

Tobacco Use, Risk Perceptions, and Characteristics of Adults Who Used a Heated Tobacco Product (IQOS) in the United States: Cross-Sectional Survey Study. [PDF]

JMIR Form Res
Cheng HG, Noggle B, Vansickel AR, Largo EG, Magnani P.   +4 more
europepmc   +1 more source

A Fusion Between Common Bean Phaseolin and the N‐Terminal Domain of Maize 16 kDa Gamma Zein: Clues for Designing Nutritionally Improved Seed Storage Proteins

Plant Biotechnology Journal, EarlyView.
ABSTRACT Increasing the nutritional value of seed storage proteins (SSPs) has been largely hindered by limited knowledge of how the features of the various SSP domains determine high accumulation in protein storage vacuoles (PSV) or as endoplasmic reticulum (ER)‐located insoluble protein bodies (PB).
Emma Costa, Lucrezia Luciani, Mahsa Taghipour Rahimi, Alessandro Vitale, Emanuela Pedrazzini   +4 more
wiley   +1 more source

A novel approach to the quantitative analysis of the particulate matter in conventional cigarette smoke and heated tobacco product aerosols. [PDF]

Heliyon
Tane EG, Martínez-Gómez L, Amorós-Pérez A, Román-Martínez MC, Lillo-Ródenas MA.   +4 more
europepmc   +1 more source