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2022
HeLa cells were embedded in Durcupan and observed with a Serial Block Face Scanning Electron Microscope (SBF SEM) following the National Centre for Microscopy and Imaging Research (NCMIR) protocol. The images were acquired with an SBF SEM 3View2XP microscope (Gatan, Pleasanton, CA, USA) attached to a Sigma VP SEM (Zeiss, Cambridge, UK).
Karabag, Cefa +1 more
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HeLa cells were embedded in Durcupan and observed with a Serial Block Face Scanning Electron Microscope (SBF SEM) following the National Centre for Microscopy and Imaging Research (NCMIR) protocol. The images were acquired with an SBF SEM 3View2XP microscope (Gatan, Pleasanton, CA, USA) attached to a Sigma VP SEM (Zeiss, Cambridge, UK).
Karabag, Cefa +1 more
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Chemical Toxicity on HeLa Cells
Current Medicinal Chemistry, 2006HeLa cells were named for Henrietta Lacks, who died in 1952 from an infection of a special type of cancer. Margaret Gey, her physician, started working with these cancer cells that are still used for medical research. In the present review, an attempt has been made to collect the data for the effects of different chemicals on HeLa cells and to discuss ...
Rajeshwar P, Verma, Corwin, Hansch
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Investigating heterogeneity in HeLa cells
Nature Methods, 2019Multi-omic study uncovers biological variation across 14 HeLa cell samples, which might help to explain the growing concerns about reproducibility issues in cell culture.
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The assembly of ribosomes in HeLa cells
Journal of Molecular Biology, 1966The assembly of proteins and RNA into a mature ribosome has been studied in the HeLa cell by examining the appearance of labeled ribosomal proteins on mature ribosomes after a pulse of radioactive amino acids followed by a chase with cold amino acids. The ribosomal proteins continue to appear as part of cytoplasmic ribosomes for a period of two hours ...
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Archiv f�r die gesamte Virusforschung, 1965
A strain of varicella virus was isolated and easily maintained in HeLa cells. The infection could be passed with live cells only, practically all infectivity being destroyed by disruption of the cells with sonication or repeated freeze-thawing, even if precautions were taken to favour the infectivity of nucleic acids.
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A strain of varicella virus was isolated and easily maintained in HeLa cells. The infection could be passed with live cells only, practically all infectivity being destroyed by disruption of the cells with sonication or repeated freeze-thawing, even if precautions were taken to favour the infectivity of nucleic acids.
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Regulation of metallothionein production in HeLa cells
Toxicology Letters, 1991Metallothioneins are cysteine-rich, heavy-metal-binding proteins which have been assumed to participate in the detoxification of toxic metals. The mechanism of thionein (apoprotein of metallothionein) induction by cadmium was studied using cultured human cells.
S, Koizumi, T, Sone
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Methylation of ribosomal proteins in HeLa cells
Archives of Biochemistry and Biophysics, 1976Abstract Methylated amino acids from both 40 and 60S subunit proteins of HeLa cytoplasmic ribosome were analyzed. It was observed that methylation of ribosomal proteins occurs in both subunits with N G , N G -dimethylarginine as the major methylated amino acid.
F N, Chang +3 more
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Chromosomal proteins of interphase HeLa cells
Biochimica et Biophysica Acta (BBA) - Protein Structure, 1970Abstract 1. 1. We describe the properties of the proteins of isolated interphase chromosomes of HeLa cells. Histones of interphase chromosomes were fractionated by chromatography on Amberlite CG-50 and further characterized by analytical disc electrophoresis and amino acid analysis of each chromatographic fraction. 2. 2.
A, Sadgopal, J, Bonner
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The metabolism of l-fucose by HeLa cells
Experimental Cell Research, 1968Abstract HeLa cells incorporate 14 C- l -fucose directly into macromolecules with no detectable randomization of 14 C-activity into other sugars. The pathway of incorporation probably proceeds via GDP- l -fucose, the concentration of which increases markedly when l -fucose is present in the growth medium.
R L, Kaufman, V, Ginsburg
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Purification and reconstitution of HeLa cell microtubules
Biochemistry, 1980Microtubules from suspension cultures of HeLa cells have been purified by carrying them through four complete cycles of polymerization at 37 degrees C and depolymerization at 4 degrees C. These microtubules show, in addition to the major alpha- and beta-tubulin components, major proteins with molecular weights of 201 000-206 000 (comprising 4.5% of the
J A, Weatherbee +2 more
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