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Hemagglutination (Inhibition) Assay
Methods in molecular biology, 2014The hemagglutination assay is a simple and easy method to obtain semi-quantitative data on the sugar binding and specificity of a lectin. An active lectin agglutinates erythrocytes by recognizing a carbohydrate on the cell surface and forming a cross-linked network in suspension.
Kotone, Sano, Haruko, Ogawa
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Hemagglutination Assay for Antigen and Antibody Associated with Viral Hepatitis
Science, 1970Hemagglutination assays are described for measuring hepatitis-associated Australia antigen and antibody. Red cells coated with isolated antigen, with chromic chloride as a coupling agent, are used for detection of antibodies. Detection of the antigen in serums depends on inhibition of hemagglutination.
G N, Vyas, N R, Shulman
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Hemagglutination Assay for the Avian Influenza Virus
Methods in molecular biology, 2008The hemagglutination (HA) assay is a tool used to screen cell culture or amnioallantoic fluid harvested from embryonating chicken eggs for hemagglutinating agents, such as type A influenza. The HA assay is not an identification assay, as other agents also have hemagglutinating properties.
M. Killian
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Diagnosis of Invasive Aspergillosis by Passive Hemagglutination Assay of Antibody
Journal of Infectious Diseases, 1980Sheep red cells treated with concanavalin A and sensitized with a partially purified aspergillus antigen were used to detect antibody to Aspergillus by passive hemagglutination (PHA). Sera from eight patients with aspergillomas or allergic aspergillosis had PHA titers of greater than or equal to 1:800 and antibody detectable by immunodiffusion (ID). Of
J W, Gold +4 more
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Development of Hemagglutination Assays
Vox Sanguinis, 1977Abstract. Conditions favoring the coupling of antibody to human erythrocytes stabilized by a variety of reagents were studied with the use of antibody to hepatitis B surface antigen. Functional anti‐HBs bound to erythrocytes was measured by radioimmune assay using 125 I‐HBsAg.
Bernard Horowitz +4 more
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Hemagglutination assay for human serum fibronectin
Journal of Immunological Methods, 1985A hemagglutination assay for human serum fibronectin (Fn) is described. The assay is based on the capacity of Fn to bind to gelatin. Purified and human serum Fn agglutinate chromic chloride treated sheep red cells coated with gelatin in a quantitative and reproducible manner.
R G, Bălună +3 more
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Hemagglutination inhibition assays
Seminars in Avian and Exotic Pet Medicine, 2002Hemagglutination is a useful characteristic of many viruses because it can provide a simple and efficient method of detecting antihemagglutinating activity in serum from exposed animals. This is called hemagglutination inhibition (HI), and HI assays are used extensively to detect and titrate antibodies to many avian diseases including Newcastle disease
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Potato lectin activity assay based on hemagglutination
Journal of the Science of Food and Agriculture, 1992AbstractA quantitative method for the determination of potato lectin activity has been developed. In the method, lectin is incubated with an excess of red blood cells (RBC) in phosphate buffered saline at 25 °C. Maximum agglutination is reached within 60 min. Agglutinated RBC are then separated from nonagglutinated RBC by filtration.
C. P. Patel, T. W. Willis
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Hemagglutination Assay For Determination Of Human Factor VIII:C
Thrombosis and Haemostasis, 1981New methods for the determination of human factor VIII clotting protein (F. VIII:C) have been developed utilizing hemagglutination technique without using any other coagulation proteins in the assay. A 50% ammonium sulphate precipitate of the plasma from a hemophilic patient containing high titer of F.
E P Kang +3 more
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Hemadsorption Plaque Assay for Hemagglutinating Encephalomyelitis Virus
American Journal of Veterinary Research, 1972SUMMARY A plaque assay was developed for titration of infectivity of hemagglutinating encephalomyelitis virus (hev). Viral infection in cell cultures was kept localized by adding nutrient medium containing 1% agar to the cultures. The medium was subsequently removed, and hemadsorption was used to detect otherwise macroscopically inapparent foci of ...
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