Results 91 to 100 of about 261,803 (304)

Analysis of BnMTL, a novel metallothionein‐like protein in the bast fiber crop Ramie (Boehmeria nivea)

open access: yesFEBS Open Bio, 2019
Ramie (Boehmeria nivea) is a perennial herb that is highly tolerant of heavy metals. In the present study, we cloned a novel metallothionein‐like gene from ramie; this gene, termed BnMTL, encodes a putative 46 amino acid protein with a molecular mass of ...
Gang Gao   +6 more
doaj   +1 more source

The hepta-β-glucoside elicitor-binding proteins from legumes represent a putative receptor family [PDF]

open access: yes, 2000
The ability of legumes to recognize and respond to β-glucan elicitors by synthesizing phytoalexins is consistent with the existence of a membrane-bound β-glucan-binding site.
Ebel, J.   +4 more
core   +1 more source

GbWAKL20 Phosphorylates GbNFYB8 to Modulate Verticillium Wilt Resistance in Cotton

open access: yesAdvanced Science, EarlyView.
Wall‐associated receptor‐like kinases (WAKLs) play pivotal roles in extracellular–intracellular signal transduction. Upon sensing Verticillium dahliae infestation at the plasma membrane, GbWAKL20 accumulates and transmits signals to the nucleus via endoplasmic reticulum‐mediated Golgi vesicle transport.
Guilin Wang   +7 more
wiley   +1 more source

MultiBac: expanding the research toolbox for multiprotein complexes [PDF]

open access: yes, 2012
This article is made available for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source.
Berger, Imre   +3 more
core   +1 more source

Genome‐Wide Codon Reprogramming Enables a Multifactorially Attenuated Influenza Vaccine with Broad Cross‐Protection

open access: yesAdvanced Science, EarlyView.
Genome‐wide codon reprogramming of influenza A virus introduces 1956 synonymous mutations across five segments and elevates CpG content, causing defective NA packaging, loss of NS1 accumulation, and enhanced ZAP‐mediated antiviral responses without affecting RNA or protein synthesis. These mechanisms result in profound attenuation and potent homologous
Yang Wang   +13 more
wiley   +1 more source

Recombinant attenuated Salmonella Typhimurium with heterologous expression of the Salmonella Choleraesuis O-polysaccharide: high immunogenicity and protection

open access: yesScientific Reports, 2017
Non-typhoidal Salmonella are associated with gastrointestinal disease worldwide and invasive disease in Africa. We constructed novel bivalent vaccines through the recombinant expression of heterologous O-antigens from Salmonella Choleraesuis in ...
Xinxin Zhao   +9 more
doaj   +1 more source

A toolbox of nanobodies developed and validated for use as intrabodies and nanoscale immunolabels in mammalian brain neurons. [PDF]

open access: yes, 2019
Nanobodies (nAbs) are small, minimal antibodies that have distinct attributes that make them uniquely suited for certain biomedical research, diagnostic and therapeutic applications.
Dong, Jie-Xian   +11 more
core  

Recombinant Envelope-Proteins with Mutations in the Conserved Fusion Loop Allow Specific Serological Diagnosis of Dengue-Infections [PDF]

open access: yes, 2015
Dengue virus (DENV) is a mosquito-borne flavivirus and a major international public health concern in many tropical and sub-tropical areas worldwide. DENV is divided into four major serotypes, and infection with one serotype leads to immunity against the
Barzon, Luisa   +5 more
core   +4 more sources

A Mini‐Spidroin Forms High‐Performance Artificial Spider Silk via Edge‐Cysteine–Locked β‐Sheet Assembly

open access: yesAdvanced Science, EarlyView.
Cysteine‐engineered mini‐spidroins (∼33 kDa) undergo phosphate‐induced liquid–liquid phase separation (LLPS), where disulfide bonds lock partially aligned polyA segments. Subsequent microfluidic spinning promotes directional β‐sheet crystallization, producing fibers with 531 MPa strength and 182 MJ/m3 toughness.
Min Li   +10 more
wiley   +1 more source

Heterologous Expression of Gene Encoded Thermostable Lipase and Lipolytic Activity

open access: yesJournal of Pure and Applied Microbiology, 2017
Three lipase genes namely LK2, LK3 and LK5 were successfully sub-cloned into expression vector pET30a. The recombinant vectors were heterologically expressed into Escherichia coli BL21 (DE3) by 1 mM IPTG induced at 37 °C.
Nurhasanah   +3 more
doaj   +1 more source

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