Results 181 to 190 of about 145,939 (263)

Development of a Universal Platform for the Heterologous Expression of Bidirectional [Ni-Fe]-Hydrogenases in <i>E. coli</i>. [PDF]

ACS Synth Biol
Siebert DL, Sargent F, Al-Shameri A, Sieber V.   +3 more
europepmc   +1 more source

Evolutionary divergence and functional insights into the heteromeric cis‐prenyltransferase of Paramecium tetraurelia

The FEBS Journal, EarlyView.
Heteromeric cis‐prenyltransferases (CPT) are indispensable for dolichol synthesis and protein N‐glycosylation in most eukaryotes. The catalytic subunits are strongly conserved throughout evolution, in contrast to the evolutionarily variable accessory subunits. The POC1 protein from Paramecium tetraurelia is the smallest identified CPT‐accessory subunit
Agnieszka Onysk, Kamil Steczkiewicz, Mariusz Radkiewicz, Paweł Link‐Lenczowski, Przemysław Surowiecki, Karolina Sztompka, Kariona A. Grabińska, Jacek K. Nowak, Liliana Surmacz   +8 more
wiley   +1 more source

Structural analysis of the NifL‐NifA complex reveals the molecular basis of anti‐activation of nitrogen fixation gene expression in Azotobacter vinelandii

The FEBS Journal, EarlyView.
Using cryo‐EM combined with biochemical and genetic approaches, we mapped the interaction surface between NifL and NifA to gain insights into the regulation of nitrogen fixation genes in A. vinelandii. Our findings suggest that NifL, a homolog of histidine kinases lacking phosphorylation activity, evolved to act as a steric block of NifA activity ...
Marcelo Bueno Batista, Jake Richardson, Michael W. Webster, Dmitry Ghilarov, John W. Peters, David M. Lawson, Ray Dixon   +6 more
wiley   +1 more source

Integrative In Silico and Experimental Characterization of Endolysin LysPALS22: Structural Diversity, Ligand Binding Affinity, and Heterologous Expression. [PDF]

Int J Mol Sci
Nawaz N, Nawaz S, Hussain A, Anayat M, Wen S, Wang F.   +5 more
europepmc   +1 more source

Heterologous plastoquinone production using a newly identified O2‐dependent cyanobacterial hydroxylase

The FEBS Journal, EarlyView.
In this study, we confirm that PlqH is the hydroxylase operating in plastoquinone biosynthesis in photosynthetic cyanobacteria (Cyanobacteriia). Our phylogenetic analyses demonstrate that cyanobacterial PlqH homologues originated from hydroxylases involved in ubiquinone biosynthesis in bacteria. Plastoquinone production in Escherichia coli was achieved
Morgane Roger‐Margueritat, Margot Beltran, Juliette Schnoebelen, Laura Flandrin, Wafa Rezali, Eline Michel, Sophie S. Abby, Fabien Pierrel   +7 more
wiley   +1 more source

Heterologous Expression and Antimicrobial Targets of a Novel Glycine-Rich Antimicrobial Peptide from <i>Artemia franciscana</i>. [PDF]

Mar Drugs
Tao M, Sun A, Shao H, Ye H, Yu G, Chen D, Zhang W.   +6 more
europepmc   +1 more source

Binding of Bacillus subtilis dynamin‐like protein DynA to the bacterial membrane is essential for effective phage defense

The FEBS Journal, EarlyView.
The bacterial dynamin‐like protein DynA from Bacillus subtilis plays a key role in phage defense. It binds the plasma membrane through a unique paddle domain in its D1 subunit. This membrane association is essential for its protective function, likely preventing explosive membrane rupture following phage infection.
Samia Shafqat, Urska Repnik, Marc Bramkamp   +2 more
wiley   +1 more source

Heterologous Expression of Bacteriocins from the Metagenome Mining of Cotija Cheese. [PDF]

Probiotics Antimicrob Proteins
Esquivel-López A, Rocha-Mendoza D, Serrano-Maldonado CE, Escobar-Zepeda A, Quirasco M.   +4 more
europepmc   +1 more source

Protein nanoparticles assemble in plants, display antigenic viral peptides, and produce an epitope‐specific immune response

The FEBS Journal, EarlyView.
Current methods to control porcine reproductive and respiratory syndrome, a disease affecting pigs, are insufficient and a safer, more effective vaccine is needed. This study describes the design and plant production of a self‐assembling protein nanoparticle vaccine candidate against this disease.
Jordan T. VanderBurgt, Richard Strasser, Hong Zhu, Angelo Kaldis, Christopher P. Garnham, Rima Menassa   +5 more
wiley   +1 more source

Heterologous expression and characterization of synthetic polyester-degrading cutinases from <i>Fusarium</i> spp. in <i>Aspergillus niger</i>. [PDF]

Microbiol Spectr
Verschoor J-A, Arentshorst M, Regensburg-Tuink AJG, Seekles SJ, van den Hondel C, de Winde JH, Ram AFJ.   +6 more
europepmc   +1 more source