Results 131 to 140 of about 4,092,880 (310)
FEBS Open Bio, EarlyView.Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn +4 more
wiley +1 more source
High-resolution traffic flow data from the urban traffic control system in Glasgow
This dataset offers a long-term traffic flow data at an intra-city scale with high spatio-temporal granularity. The dataset covers the Glasgow City Council area for four consecutive years spanning the COVID-19 pandemic, from October 2019 to September ...
Wang, Mingshu, Li, Yue, Zhao, Qunshan
core +1 more source
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Efficient algorithm for testing goodness-of-fit for classification of high dimensional data
Lietuvos Matematikos Rinkinys, 2009 Let us have a sample satisfying d-dimensional Gaussian mixture model (d is supposed to be large). The problem of classification of the sample is considered. Because of large dimension it is natural to project the sample to k-dimensional (k = 1, 2, . . .)
Gintautas Jakimauskas
doaj +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Feature Selection for High Dimensional Data Using Monte Carlo Tree Search
IEEE Access, 2018 Feature selection is the preliminary step in machine learning and data mining. It identifies the most important and relevant features within a dataset by eliminating the redundant or irrelevant features.
Muhammad Umar Chaudhry, Jee-Hyong Lee
doaj +1 more source
FEBS Open Bio, EarlyView.Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman +4 more
wiley +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Estimating High Dimensional Covariance Matrices and its Applications [PDF]
Estimating covariance matrices is an important part of portfolio selection, risk management, and asset pricing. This paper reviews the recent development in estimating high dimensional covariance matrices, where the number of variables can be greater ...
Jushan Bai, Shuzhong Shi
core
FEBS Open Bio, EarlyView.Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel +8 more
wiley +1 more source