Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
HCNet: Multi-Exposure High-Dynamic-Range Reconstruction Network for Coded Aperture Snapshot Spectral Imaging. [PDF]
Shi H, Chen J, Li Y, Zhang P, Tian J.
europepmc +1 more source
Fringe Projection Profilometry Based on Saturated Fringe Restoration in High Dynamic Range Scenes. [PDF]
Li H +7 more
europepmc +1 more source
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Performance Characterization and Tuning of a Charge-Splitting High Dynamic Range 4-Tap CMOS Image Sensor. [PDF]
Feng Y +4 more
europepmc +1 more source
High-Dynamic-Range Spectral Reflectance for the Segmentation of Paint Pigment: Application to Dalí's Oil Painting Dos Figuras (1926). [PDF]
Fernandez-Balbuena AA +6 more
europepmc +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
An Adaptive Clear High-Dynamic Range Fusion Algorithm Based on Field-Programmable Gate Array for Real-Time Video Stream. [PDF]
Huang H, Xu Y, Zhao T.
europepmc +1 more source
Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum
Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik +6 more
wiley +1 more source
Multicenter evaluation of label-free quantification in human plasma on a high dynamic range benchmark set. [PDF]
Distler U +38 more
europepmc +1 more source

