Results 161 to 170 of about 412,226 (305)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Computational single-image high dynamic range imaging

open access: yes, 2018
This thesis proposes solutions for increasing the dynamic range (DR)—the number of intensity levels—of a single image captured by a camera with a standard dynamic range (SDR). The DR in a natural scene is usually too high for SDR cameras to capture, even
Rouf, Mushfiqur
core  

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

open access: yesFEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui   +10 more
wiley   +1 more source

Image with high dynamic range

open access: yes, 2011
Tato práce se zabývá vysvětlením principu vzniku a zpracování obrazu s vysokým dynamickým rozsahem. Jsou zde popsány techniky jeho snímání, zobrazování a využití.
Láněk, Jan
core  

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

High dynamic range image processing

open access: yes, 2013
High Dynamic Range (HDR) image has an increasingly popular demand nowadays. A wide range of methods have been developed to achieve the best result. The development focuses on upgrading the quality of standard JPEG image into HDR. The most common approach
Aloysius Adi Pratama
core  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

open access: yesFEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel   +8 more
wiley   +1 more source

Towards Joint Super-Resolution and High Dynamic Range Image Reconstruction

open access: yes, 2013
The main objective for digital image- and video camera systems is to reproduce a real-world scene in such a way that a high visual quality is obtained.
Bengtsson, Tomas
core  

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