Results 141 to 150 of about 33,172 (312)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Uninterruptible Power Supply (UPS) using high frequency transformer [PDF]

open access: yes, 2010
Uninterruptible power supplies (UPS) design with low frequency transformers for isolation purposes are known to contribute to their bulkiness in terms of size and weight.
Pil Ramli, Suzana
core  

Broadband Transfer Characteristics of DC Electronic Current Transformers

open access: yesIEEE Access
The frequent operation of the switching devices inside the converter valve (mainly composed of thyristors) and other equipment in the converter station of ultra-high voltage direct current (UHVDC) transmission system generates a large number of high ...
Songyu Yang   +3 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

94 GHz Monolithic Transmitter for Weather Radar Application [PDF]

open access: yes, 2011
This thesis was written for concluding my studies at the University of Padua. The main topic is the design of a monolithic transmitter in SiGe bipolar technology, for weather radar application at an operating frequency around 94GHz.
Puliero , Claudio
core  

Power Electronic Transformer Using DC-DC Converter Based on Energy Injection Control

open access: yesKongzhi Yu Xinxi Jishu, 2017
In order to improve the power density and efficiency of power electronic transformer, this paper presented a power electronic transformer using DC-DC converter based on energy injection control.
ZHAO Ziyi, SU Mei, ZHU Qi, WANG Hui
doaj  

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Retificador buck trifásico unidirecional PWM isolado em alta-frequência por único estágio [PDF]

open access: yes, 2012
Tese (doutorado) - Universidade Federal de Santa Catarina, Centro Tecnológico, Programa de Pós-Graduação em Engenharia Elétrica, Florianópolis, 2009Descreve-se uma nova topologia de conversor CA-CC trifásico isolado em alta-frequência por único estágio ...
Greff, Diego Santos
core  

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

open access: yesFEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel   +4 more
wiley   +1 more source

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