Results 181 to 190 of about 20,817,051 (316)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Lesion Depth Optimization in High-Power Radiofrequency Ablation: Evaluating Single High-Power and Combined Very High-Power Applications. [PDF]

J Cardiovasc Electrophysiol
Iwakawa H, Takigawa M, Kato R, Yamaguchi J, Honda M, Tateishi R, Negishi M, Kawamura I, Goto K, Nishimura T, Yamao K, Tao S, Suzuki S, Iwanaga T, Miyazaki S, Watanabe H, Sasano T.   +16 more
europepmc   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Intensification of lupin protein solubilization using high-power ultrasound. [PDF]

Ultrason Sonochem
Navarro-Vozmediano P, Benedito J, Domínguez-Valencia R, Blay MT, Garcia-Perez JV.   +4 more
europepmc   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

General photonic-crystal slab cavity design for scalable high-power single-mode lasing. [PDF]

Light Sci Appl
Zhou M, Pan M, Gautam C, Seth S, Rotter T, Balakrishnan G, Zhou W, Fan S.   +7 more
europepmc   +1 more source

BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells

FEBS Open Bio, EarlyView.
BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley   +1 more source

Suppressing acoustomigration and temperature rise for high-power robust acoustics. [PDF]

Nat Commun
Qian F, Chen S, Wei W, Xu J, Yang K, Zheng J, Ren Z, Liu X, Yang Y.   +8 more
europepmc   +1 more source

Genetic dissection of human ABCE1 in yeast reveals separable requirements for ribosome recycling and suppression of aberrant reinitiation

FEBS Open Bio, EarlyView.
Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata, Yuxin Li, Kei Endo, Koichi Ito   +3 more
wiley   +1 more source

A Framework for Short-Range Wireless Power and Data Transfer in Miniaturized High-Power, High-Bandwidth Implants. [PDF]

Micromachines (Basel)
Ramaut L, Polfliet P, Callebaut G, Van der Perre L.   +3 more
europepmc   +1 more source