Results 261 to 270 of about 225,502 (309)

High pressure liquid chromatography of neutral glycosphingolipids

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1972
Abstract A system for the separation of neutral glycosphingolipids by high pressure liquid chromatography of their benzoylated derivatives has been devised. Serum mono-, di-, tri-and tetraglycosylceramides are completely resolved within 25 min. A full scale recorder response can be obtained with approximately 1.0 nmole of glycolipid.
J E, Evans, R H, McCluer
openaire   +2 more sources

Anthracycline Assay by High-pressure Liquid Chromatography

Journal of Pharmaceutical Sciences, 1981
A general method of analysis of anthracycline concentrations was developed. Drug is extracted from plasma with organic solvent and separated from metabolites by high-pressure liquid chromatography on an aminocyanosilica column. Detection and quantitation are by the endogenous fluorescence of compounds having an intact tetracyclic ring structure. Limits
S D, Averbuch, T T, Finkelstein, S E, Fandrich, S D, Reich   +3 more
openaire   +2 more sources

Assay of Cefotaxime by High-Pressure-Liquid Chromatography

Chemotherapy, 2009
A high-pressure-liquid chromatographic (HPLC) procedure for quantitative assay of cefotaxime (CT) and its major metabolite in serum of normal individuals, desacetyl cefotaxime (DACT), is described. It employs Lichrosorb RP-8, elution with phosphoric-acid-methanol and UV absorption at 310 nm.
T, Bergan, R, Solberg
openaire   +2 more sources

Mexiletine in Plasma by High Pressure Liquid Chromatography

Therapeutic Drug Monitoring, 1981
We report here a novel high pressure liquid chromatographic (HPLC) technique for use in therapeutic monitoring of the primary amine antiarrhythmic drug mexiletine in plasma samples. Mexiletine and its structural analogue, the internal standard Ko 768, are extracted with heptane after alkalinization of the sample. The extracts are evaporated to dryness,
R, Kelly, D, Christmore, R, Smith, L, Doshier, S L, Jacobs   +4 more
openaire   +2 more sources

High-pressure liquid chromatography of steroidal alkaloids

Journal of Chromatography A, 1976
High-pressure liquid chromatography was used to separate the following steroidal alkaloids: tomatidine, solanidine, solasodine, rubijervine, veratramine and jervine. The method was used to prepare crystalline solanidine from a crude mixture of aglycones obtained from Solanum chacoense, and to separate radioactive solanidine from extracts of potato ...
I R, Hunter, M K, Walden, J R, Wagner, E, Heftmann   +3 more
openaire   +2 more sources

Determination of hydroxyproline by high pressure liquid chromatography

Analytical Biochemistry, 1992
A rapid, precise, and simple HPLC method provides an assay of hydroxyproline from tissue extracts or solutions of collagen. Samples are hydrolyzed with 6 N HCl, derivatized with phenyl isothiocyanate, and chromatographed on a small, C18 reverse-phase HPLC column. Hydroxyproline (Hyp) is separated from other amino acids and detected by absorption at 254
G D, Green, K, Reagan
openaire   +2 more sources

Determination of Thiopental by High Pressure Liquid Chromatography

Acta Pharmacologica et Toxicologica, 1979
AbstractA specific and rapid reverse phase HPLC‐method for the determination of thiopental in serum and in protein free solutions is described. No extraction is used. The detection limit is about 0.090 μg/ml for serum and about 0.030 μg/ml for buffer solutions. The retention time is 3.80 min.
J H, Christensen, F, Andreasen
openaire   +2 more sources

A fluorescence detector for high-pressure liquid chromatography

Journal of Chromatography A, 1977
We describe a fluorescence spectrophotometer adapted with a micro quartz flow cell to record the output of modern liquid chromatographs. The optical system is double beam in that the light source variations are cancelled out by a second photomultiplier, thus enhancing the sensitivity of the technique. The emission spectra may be scanned by stopping the
W, Slavin, A T, Williams, R F, Adams
openaire   +2 more sources

Separation of cytokinins by High Pressure Liquid Chromatography

Planta, 1975
A number of cytokinin reference compounds have been successfully separated by High Pressure Liquid Chromatography using columns of pellicular strong cation exchange resin and of pellicular polyamide. On polyamide, all cytokinins were eluted within 10 min with an aqueous buffer but on the cation exchange resin some cytokinins (generally those with bulky
openaire   +2 more sources

High-pressured liquid chromatography of carotenoids

Journal of Chromatography A, 1978
Abstract The potential of high-pressure liquid chromatography (HPLC) for separation of carotenoids on silica has been studied with selected model carotenoids (carotenes, diols, cis—trans isomers and diastereoisomers) in comparison with conventional thin-layer chromatography and circular paper techniques.
Anne Fiksdahl, Julius T. Mortensen, Synnøve Liaaen-Jensen   +2 more
openaire   +1 more source