Results 91 to 100 of about 19,221,438 (289)

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Arts et éducation relative à l’environnement : un maillage incontournable [PDF]

, 2017
L’histoire fourmille d’exemples où le pouvoir mobilisateur et transformateur de l’art a servi différentes causes sociales, politiques, religieuses, voire commerciales.
Bouchard-Valentine, Vincent
core   +1 more source

Gaussian Markov Random Fields over graphs of paths and high relative accuracy

Journal of Computational and Applied Mathematics
The present paper presents some results that allow us to perform with High Relative Accuracy linear algebra operations with correlation and covariance matrices of Gaussian Markov Random Fields over graphs of paths. Some numerical experiments are carried out showing the computational benefits of this approach.
Juan Baz, Pedro Alonso 0001, Juan Manuel Peña 0001, Raúl Pérez-Fernández   +3 more
openaire   +6 more sources

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Two-Way Single-Photon Laser Time Transfer for High-Speed Moving Platforms

Photonics
The two-way laser time transfer technology, based on single-photon detection, is among the techniques requiring the least weight and power consumption for ultra-long-distance clock synchronization.
Xinyi Zhu, Yurong Wang, Zhaohui Li, Xue Li, Guang Wu   +4 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

The NAVSCIN Project: Towards High-Accuracy Navigation under Scintillation [PDF]

, 2019
The main goal of the Marie Sklodowska-Curie Action (MSCA) titled “High Accuracy Navigation under Scintillation Conditions (NAVSCIN)” is to develop an improved strategy to mitigate scintillation –a particular type of space weather perturbation–tailored ...
Juan Zornoza, José Miguel, Rovira Garcia, Adrià, González Casado, Guillermo, Escudero Royo, Miguel, Sanz Subirana, Jaume   +4 more
core   +1 more source

High relative accuracy for rational q-Bernstein–Vandermonde matrices

Linear and Multilinear Algebra
In this article, an accurate and efficient method to compute the eigenvalues, singular values and inverses of nonsingular totally positive rational q-Bernstein–Vandermonde matrices and the solution of some associated linear systems of equations is provided.
Delgado, Jorge, Orera, Héctor, Peña, J.M.   +2 more
openaire   +3 more sources