Results 81 to 90 of about 24,544,639 (252)

CRISPR-Cas Technology for Constructing Engineered Strains to Produce High Value-Added Industrial Products [PDF]

open access: yesBIO Web of Conferences
With its ability to make precise, effective, and programmable genetic changes, the CRISPR-Cas system has become a revolutionary tool for microbial genome engineering. This article summarizes the use of CRISPR-Cas technology to create modified strains for
Teng Aiyoujia
doaj   +1 more source

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

open access: yesFEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav   +2 more
wiley   +1 more source

Research on High Effi ciency Computing Technology Based on COTS Products

open access: yes天地一体化信息网络, 2020
Aiming at the problem of space environment adaptability of COTS products,the fault tolerance reinforcement of space computer was carried out through crosss layer collaborative software fault tolerant technology.The space computer based on COTS product ...
Yue ZHANG   +4 more
doaj   +2 more sources

From junk to function — How weak selection in eukaryotes builds new parts and drives genomic complexity

open access: yesFEBS Letters, EarlyView.
How do genomes gain new functional parts? In eukaryotes, which tend to evolve under weak selection, much of the genome is junk. Palazzo and Qiu borrow the logic of Markov chains to show how non‐functional DNA becomes functional through the appearance of intermediate states, which arise due to epistasis, buffering, and biochemical messiness, allowing ...
Alexander F. Palazzo, Yi Qiu
wiley   +1 more source

The internationalisation of British start-up companies in high-technology industries [PDF]

open access: yes
The present thesis analyses the international activities of British start-up companies in high-technology industries. The research makes the following contributions.
Burgel, Oliver
core  

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

open access: yesFEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge   +6 more
wiley   +1 more source

The Center for Sustainable Technology at Tech High: A Vision of Sustainability Education in Atlanta

open access: yes, 2009
Presented on March 3, 2009 from 6:00 pm to 7:30 pm in the Stamps Student Center Commons Piedmont RoomSponsored by the School of Public Policy and Students Organizing for Sustainability.Runtime: 74:48 minutesAlan Gravitt presented a talk about high school
Gravitt, Alan
core  

Technology Architectural Review [PDF]

open access: yes, 1887
Publication of the Massachusetts Institute of Technology Architectural Society. Volume 3.
Massachusetts Institute of Technology. Department of Architecture   +1 more
core  

Prospecting the protein design landscape

open access: yesFEBS Letters, EarlyView.
This review outlines the current state of various protein design approaches. We discuss the current possibilities enabled by recently released tools, highlight future avenues to pursue in protein design, and underscore the crucial role of key databases and resources for successful protein design workflows.
Jakob R. Riccabona   +4 more
wiley   +1 more source

L‐aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in Synechocystis sp. PCC6803

open access: yesFEBS Letters, EarlyView.
Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil   +3 more
wiley   +1 more source

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