Results 81 to 90 of about 1,799,132 (267)
Environmental and emotional self-regulation skills play a critical role in promoting well-being of individuals and in encouraging healthy relationships.
Viola Sallay +3 more
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Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj +3 more
wiley +1 more source
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley +1 more source
Being-at-Home. Winnicott, Levinas, and Bachelard [PDF]
The paper investigates the concept of being-at-home in both phenomenology and psychoanalysis. In order to accomplish this task, I draw on Donald Winnicott’s scattered psychoanalytical observations regarding the question what belonging to a home means ...
David-Augustin Mândruț
doaj +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
RESUMEN: La pintura es una fuente esencial para la historia y un elemento revelador para la investigación sobre el papel de la mujer en la sociedad a lo largo de la historia.
Rafael Matas Fernandez +1 more
doaj
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro +1 more
wiley +1 more source
Jean Barbier-en Piarres XX. mendearen lehen erdiko euskal eleberrigintzan [PDF]
Jean Barbierren Piarres I (1926) eta Piarres II (1929) idazlanak aztertzen dira, XX. mendearen lehen erdiko euskal eleberrigintzan kokatuz. XIX. mendearen amaieratik, mende erdi luzean argitaratutako eleberri guztiak bat datoz erabilitako langaiak ...
Ana M. Toledo
doaj

