Results 121 to 130 of about 345,396 (332)

BLOC1S1 Attenuates B. Melitensis 16M LPS‐Triggered Autophagy by Spatial Confinement of TDP‐43

Advanced Science, EarlyView.
This study explores how B. melitensis 16M LPS and BLOC1S1 regulate autophagy in goat spermatogonial stem cells. The 16M LPS is activating AMPK‐dependent autophagy, increasing LC3B‐II/I ratios and lysosomal genes, while Escherichia coli LPS shows no effect.
Shicheng Wan, Miao Han, Mengfei Zhang, Wenbo Chen, Fangde Xie, Xuan Luo, Wenping Wu, Congliang Wang, Donghui Yang, Bin Han, Haijing Zhu, Haisheng Yu, Na Li, Jinlian Hua   +13 more
wiley   +1 more source

Rapid in vitro method to assemble and transfer DNA fragments into the JCVI-syn3B minimal synthetic bacterial genome through Cre/loxP system

Biophysics and Physicobiology
JCVI-syn3B (syn3B), a minimal synthetic bacterium that only possesses essential genes, facilitates the examination of heterogeneous gene functions in minimal life. Conventionally, Escherichia coli is used to construct DNA fragments for gene transfer into
Atsuko Uenoyama, Hana Kiyama, Mone Mimura, Makoto Miyata   +3 more
doaj   +1 more source

Oligonucleotide-Mediated, PCR-Independent Cloning by Homologous Recombination

BioTechniques, 2001
We have developed an oligonucleotide-mediated cloning technique based on homologous recombination in Saccharomyces cerevisiae that allows precise DNA sequences to be transferred independent of restriction enzymes and PCR.
D.J. DeMarini, C.L. Creasy, Q. Lu, J. Mao, S.A. Sheardown, G.M. Sathe, G.P. Livi   +6 more
doaj   +1 more source

Mitotic antipairing of homologous and sex chromosomes via spatial restriction of two haploid sets. [PDF]

, 2018
Pairing homologous chromosomes is required for recombination. However, in nonmeiotic stages it can lead to detrimental consequences, such as allelic misregulation and genome instability, and is rare in human somatic cells.
Hua, Lisa L, Mikawa, Takashi
core   +1 more source

Biosynthesis of H2S and Siderophores Targeting Gram‐Negative Bacterial Resistance to Reactive Oxygen Species

Advanced Science, EarlyView.
Discover how Gram‐negative bacteria develop resistance to reactive oxygen species, and how scientists aim to overcome this challenge. Abstract Reactive oxygen species (ROS) are a promising alternative bactericide. However, it is questioned that bacteria can potentially develop resistance to ROS, similar to their resistance against antibiotics and ...
Congyang Mao, Wanyu Jin, Yiming Xiang, Yizhou Zhu, Jun Wu, Xiangmei Liu, Shuilin Wu, Wei Qiao, Kenneth M. C. Cheung, Kelvin Wai Kwok Yeung   +9 more
wiley   +1 more source

A cell cycle-dependent BRCA1–UHRF1 cascade regulates DNA double-strand break repair pathway choice

Nature Communications, 2016
BRCA1 is a key regulator of DNA double-strand break repair, functioning to promote homologous recombination and repress non-homologous end-joining. Here the authors show that the ubiquitin ligase UHRF1 is recruited to breaks by BRCA1, where it targets ...
Haoxing Zhang, Hailong Liu, Yali Chen, Xu Yang, Panfei Wang, Tongzheng Liu, Min Deng, Bo Qin, Cristina Correia, Seungbaek Lee, Jungjin Kim, Melanie Sparks, Asha A. Nair, Debra L. Evans, Krishna R. Kalari, Pumin Zhang, Liewei Wang, Zhongsheng You, Scott H. Kaufmann, Zhenkun Lou, Huadong Pei   +20 more
doaj   +1 more source

CRISPR/Cas Gene Editing of a Large DNA Virus: African Swine Fever Virus

Bio-Protocol, 2018
Gene editing of large DNA viruses, such as African swine fever virus (ASFV), has traditionally relied on homologous recombination of a donor plasmid consisting of a reporter cassette with surrounding homologous viral DNA.
Manuel Borca, Keith Berggren, Elizabeth Ramirez-Medina, Elizabeth Vuono, Douglas Gladue   +4 more
doaj   +1 more source

Stimulation of recombination between homologous sequences on plasmid DNA and chromosomal DNA in Escherichia coli by N-acetoxy-2-acetylaminofluorene. [PDF]

, 1984
C Luisi-DeLuca, Ronald D. Porter, William D. Taylor   +2 more
openalex   +1 more source

CLAE: A High‐Fidelity Nanopore Sequencing Strategy for Read‐Level Viral Variant Detection and Environmental RNA Virus Discovery

Advanced Science, EarlyView.
High‐fidelity Nanopore sequencing offers a cost‐effective, portable path to uncovering viral dark matter in complex environments—but suffers from severe read‐length bias, low throughput, and limited accuracy. This study traces these limitations to core biochemical barriers and introduces CLAE, a foundational high‐fidelity platform that enables accurate,
Hannah Yu, Sarah Golconda, Ga‐Eun Lee, Dantong Xue, Guillermo Domínguez‐Huerta, James M. Wainaina, Benjamin Bolduc, Shashanka Murthy, Shihyoung Kim, Seth Faith, Shan‐Lu Liu, Jiyoung Lee, Michael Oglesbee, Matthew B Sullivan, Sanggu Kim   +14 more
wiley   +1 more source

Targeting RBM10‐Repressed RORB Activity in Liquid Condensates Inhibits Lysosomal Biogenesis and Neuroblastoma Progression via Affecting NF‐κB Signaling

Advanced Science, EarlyView.
This study identifies that RBM10 represses the transcriptional activity of RORB in liquid condensates, resulting in down‐regulation of NR1D1 and RIOK3 that further activates NF‐κB activity to facilitate lysosomal biogenesis and aggressive features of NB cells.
Yanhua Guo, Xiaojing Wang, Chunhui Yang, Zhijie Wang, Xiaolin Wang, Xinyue Li, Jiaying Qu, Shunchen Zhou, Liduan Zheng, Qiangsong Tong   +9 more
wiley   +1 more source