Results 141 to 150 of about 766,679 (374)

Reappearance from Obscurity: Mammalian Rad52 in Homologous Recombination

open access: yesGenes, 2016
Homologous recombination (HR) plays an important role in maintaining genomic integrity. It is responsible for repair of the most harmful DNA lesions, DNA double-strand breaks and inter-strand DNA cross-links.
Kritika Hanamshet, O. Mazina, A. Mazin
semanticscholar   +1 more source

From Code to Life: The AI‐Driven Revolution in Genome Editing

open access: yesAdvanced Science, EarlyView.
This review explores AI‐driven advancements in genome editing, focusing on CRISPR optimization, enhanced targeting precision, reduced off‐target effects, and novel tool design. It addresses ethical challenges, data biases, and highlights future prospects in precision medicine, agriculture, and synthetic biology.
Zhidong Li   +10 more
wiley   +1 more source

Small RNA Toxin‐Assisted Evolution of GC‐Preferred ErCas12a for Enhanced Genome Targeting Range

open access: yesAdvanced Science, EarlyView.
ErCas12a is engineered to target GC‐rich PAMs using a small RNA toxin‐aided positive screening system. The resulting variant, enErCas12a, exhibits an expanded PAM profile and facilitates efficient gene editing in both bacterial and mammalian cells, while preserving high targeting specificity for both canonical and non‐canonical PAM targets.
Zehua Chen   +8 more
wiley   +1 more source

A change of view: homologous recombination at single-molecule resolution

open access: yesNature reviews genetics, 2017
Genetic recombination occurs in all organisms and is vital for genome stability. Indeed, in humans, aberrant recombination can lead to diseases such as cancer.
Kyle Kaniecki, L. Tullio, E. Greene
semanticscholar   +1 more source

A Two‐Protein Chemoreceptor Complex Regulates Oxygen Thresholds in Bacterial Magneto‐Aerotaxis

open access: yesAdvanced Science, EarlyView.
Magnetotactic bacteria align with Earth's magnetic field, helping them migrate toward low‐oxygen environments. This study reveals how Magnetospirillum gryphiswaldense uses a specialized flavin adenine dinucleotide (FAD)‐dependent chemoreceptor, composed of two interacting proteins, for precise navigation in oxygen gradients.
Julian Herz   +13 more
wiley   +1 more source

Oligonucleotide-Mediated, PCR-Independent Cloning by Homologous Recombination

open access: yesBioTechniques, 2001
We have developed an oligonucleotide-mediated cloning technique based on homologous recombination in Saccharomyces cerevisiae that allows precise DNA sequences to be transferred independent of restriction enzymes and PCR.
D.J. DeMarini   +6 more
doaj   +1 more source

An Anisotropic Gold‐Palladium Heterostructured Nanosystem for Synergistically Overcoming Radioresistance and Enhancing Melanoma Radioimmunotherapy

open access: yesAdvanced Science, EarlyView.
A multifunctional anisotropic Au‐Pd heterostructured nanosystem is developed that incorporates a plasmonically enhanced Au‐Pd core, with a shell composed of a biodegradable, Mn‐doped targeting peptide. The nanosystem integrates photothermal, radiotherapeutic, and immunomodulatory functions.
Cheng Chen   +8 more
wiley   +1 more source

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