Results 151 to 160 of about 345,396 (332)

Efficient Gene Targeting Mediated by Adeno-Associated Virus and DNA Double-Strand Breaks [PDF]

, 2003
Gene targeting is the in situ manipulation of the sequence of an endogenous gene by the introduction of homologous exogenous DNA. Presently, the rate of gene targeting is too low for it to be broadly used in mammalian somatic cell genetics or to cure ...
Baltimore, David, Cathomen, Toni, Porteus, Matthew H., Weitzman, Matthew D.   +3 more
core  

Multimodal Imaging Reveals Cerebral Microcirculation Dynamics and Mechanisms in Mouse Central Nervous System After Intranasal Infection With Recombinant Pseudorabies Virus

Advanced Science, EarlyView.
Multi‐modality imaging strategy applied to PRV‐induced central nervous system infection enables dynamic visualization of cerebrovascular dysfunction, including hyperemia, hypoperfusion, and vascular remodeling. These changes are linked to microglial activation and impaired angiogenesis, providing mechanistic insight into PRV neuropathogenesis and ...
Shuting Ling, Chongxin Wu, Mengxuan Gui, Kaiyun Chen, Yanbo Yang, Jiwei Xing, Fengxian Du, Wei Liao, Luyao Yang, Zhaokui Jin, Ningshao Xia, Guosong Wang, Yixin Chen, Qingliang Zhao   +13 more
wiley   +1 more source

Effect of double-strand breaks on homologous recombination in mammalian cells and extracts. [PDF]

, 1985
K Y Song, Lavanya Chekuri, Sikha Rauth, S. Dusko Ehrlich, Raju Kucherlapati   +4 more
openalex   +1 more source

Smohaze‐Upregulated RFWD3 Competes with TRIM24 to Stabilize TREX1 and Reduce Cytosolic dsDNA in Non‐Small Cell Lung Cancer

Advanced Science, EarlyView.
The E3 ubiquitin ligase RFWD3 binds the exonuclease TREX1 and protects it from TRIM24‐mediated ubiquitination and degradation, leading to the eradication of intracellular dsDNA, inhibition of the cGAS‐STING pathway, and immunosuppressive microenvironment of lung cancer.
Xue‐Yan Shi, Yu‐Ke Shen, Meng‐Yao Lv, Yu Sun, Yong‐Fang Lin, Zheng Wang, Xiao‐Liang Jie, Zheng Liu, Yang‐Tong Liu, Yang‐Xin Fu, Zhenhua Ren, Gui‐Zhen Wang, Guang‐Biao Zhou   +12 more
wiley   +1 more source

A Porcine Monoclonal Antibody Targeting a Conserved GP4 Epitope Protects against In Vivo Infection via the Induction of Broad‐Spectrum PRRSV Neutralization

Advanced Science, EarlyView.
This study developed mAb‐5F2, a monoclonal antibody targeting conserved GP4 residues (Q48/I50), which blocks the GP4‐CD163 interaction, resulting in a 78–85% reduction in viral entry. Porcineized 5F2‐pFc prevented mortality in infected piglets, reduced pulmonary viral loads, and minimized lesions. This broad‐spectrum antibody platform overcomes vaccine
Xu Chen, Jiakai Zhao, Pinpin Ji, Xiaoxuan Li, Hui Niu, Dian Jiao, Lu Zhang, Qianyi Zhu, Xinyu Liu, Julian A. Hiscox, James P. Stewart, Yani Sun, Qin Zhao   +12 more
wiley   +1 more source

Germ-line transmission of a planned alteration made in a hypoxanthine phosphoribosyltransferase gene by homologous recombination in embryonic stem cells. [PDF]

, 1989
Beverly H. Koller, Lora J. Hagemann, Thomas Doetschman, John R. Hagaman, Shaobai Huang, Parker Williams, N. L. First, Nobuo Maeda, O. Smithies   +8 more
openalex   +1 more source

MoMtg1 Acts as a Novel Transcriptional Repressor of MoSwi6 During Appressorium‐Mediated Penetration in the Rice Blast Fungus

Advanced Science, EarlyView.
Appressoria are specialized penetration structures for many plant pathogenic fungi, demanding precise cell cycle control. This study unveils MoMtg1 as a transcriptional repressor of MoSwi6, orchestrating MoCYC1 expression during turgor‐driven cell cycle progression to enable appressorium‐mediated penetration. Moreover, a small‐molecule inhibitor ZS1619
Xingyu Wang, Ting Zhang, Qi Li, Chang‐An Ji, Lin Huang, Mingzhi Zhang, Leiyun Yang, Xinyu Liu, Muxing Liu, Gang Li, Zhengguang Zhang, Haifeng Zhang   +11 more
wiley   +1 more source

Homologous pairing in genetic recombination: complexes of recA protein and DNA.

, 1979
Takahiro Shibata, Richard P. Cunningham, Chandan Dasgupta, Charles M. Radding   +3 more
openalex   +1 more source

Homologous alpha satellite sequences on human acrocentric chromosomes with selectivity for chromosomes 13, 14 and 21: implications for recombination between nonhomologues and Robertsonian translocations [PDF]

, 1988
K. H. Andy Choo, Bryce Vissel, Robert Brown, R. GAY FILBY, Elizabeth D. Earle   +4 more
openalex   +1 more source

HTGTS‐TCR‐Seq for Profiling of Mouse and Human T‐Cell Receptor α and β Gene Rearrangements and Diversity

Advanced Science, EarlyView.
HTGTS‐TCR‐seq is an efficient and cost‐effective method for profiling TCRα and TCRβ rearrangements using only a few primers. It captures both productive and nonproductive recombination events, minimizes primer bias, and reveals stage‐specific and age‐related repertoire changes in mice, as well as conserved features in human peripheral T cells ...
Rui Luo, Yawei Song, Meichen Wang, Longhao Zou, Fangtai Jiao, Tiange Yang, Guangchuan Wang, Zhuoyi Liang, Wei Wu, Hai‐Qiang Dai   +9 more
wiley   +1 more source