Results 151 to 160 of about 773,827 (377)

A cell cycle-dependent BRCA1–UHRF1 cascade regulates DNA double-strand break repair pathway choice

Nature Communications, 2016
BRCA1 is a key regulator of DNA double-strand break repair, functioning to promote homologous recombination and repress non-homologous end-joining. Here the authors show that the ubiquitin ligase UHRF1 is recruited to breaks by BRCA1, where it targets ...
Haoxing Zhang, Hailong Liu, Yali Chen, Xu Yang, Panfei Wang, Tongzheng Liu, Min Deng, Bo Qin, Cristina Correia, Seungbaek Lee, Jungjin Kim, Melanie Sparks, Asha A. Nair, Debra L. Evans, Krishna R. Kalari, Pumin Zhang, Liewei Wang, Zhongsheng You, Scott H. Kaufmann, Zhenkun Lou, Huadong Pei   +20 more
doaj   +1 more source

CRISPR/Cas Gene Editing of a Large DNA Virus: African Swine Fever Virus

Bio-Protocol, 2018
Gene editing of large DNA viruses, such as African swine fever virus (ASFV), has traditionally relied on homologous recombination of a donor plasmid consisting of a reporter cassette with surrounding homologous viral DNA.
Manuel Borca, Keith Berggren, Elizabeth Ramirez-Medina, Elizabeth Vuono, Douglas Gladue   +4 more
doaj   +1 more source

Tuberculosis vaccine strain _Mycobacterium bovis_ BCG Russia is a natural _recA_ mutant [PDF]

, 2008
The current tuberculosis vaccine is a live vaccine derived from _Mycobacterium bovis_ and attenuated by serial _in vitro_ passaging. All vaccine substrains in use stem from one source, strain Bacille Calmette-Guérin.
Erik C. Boettger, Peter M. Keller, Peter Sander   +2 more
core   +1 more source

Homologous sequences other than insertion elements can serve as recombination sites in plasmid drug resistance gene amplification [PDF]

, 1983
Brian C. Peterson, R H Rownd
openalex   +1 more source

CLAE: A High‐Fidelity Nanopore Sequencing Strategy for Read‐Level Viral Variant Detection and Environmental RNA Virus Discovery

Advanced Science, EarlyView.
High‐fidelity Nanopore sequencing offers a cost‐effective, portable path to uncovering viral dark matter in complex environments—but suffers from severe read‐length bias, low throughput, and limited accuracy. This study traces these limitations to core biochemical barriers and introduces CLAE, a foundational high‐fidelity platform that enables accurate,
Hannah Yu, Sarah Golconda, Ga‐Eun Lee, Dantong Xue, Guillermo Domínguez‐Huerta, James M. Wainaina, Benjamin Bolduc, Shashanka Murthy, Shihyoung Kim, Seth Faith, Shan‐Lu Liu, Jiyoung Lee, Michael Oglesbee, Matthew B Sullivan, Sanggu Kim   +14 more
wiley   +1 more source

Rapid in vitro method to assemble and transfer DNA fragments into the JCVI-syn3B minimal synthetic bacterial genome through Cre/loxP system

Biophysics and Physicobiology
JCVI-syn3B (syn3B), a minimal synthetic bacterium that only possesses essential genes, facilitates the examination of heterogeneous gene functions in minimal life. Conventionally, Escherichia coli is used to construct DNA fragments for gene transfer into
Atsuko Uenoyama, Hana Kiyama, Mone Mimura, Makoto Miyata   +3 more
doaj   +1 more source

Homologous alpha satellite sequences on human acrocentric chromosomes with selectivity for chromosomes 13, 14 and 21: implications for recombination between nonhomologues and Robertsonian translocations [PDF]

, 1988
K. H. Andy Choo, Bryce Vissel, Robert Brown, R. GAY FILBY, Elizabeth D. Earle   +4 more
openalex   +1 more source

Targeting RBM10‐Repressed RORB Activity in Liquid Condensates Inhibits Lysosomal Biogenesis and Neuroblastoma Progression via Affecting NF‐κB Signaling

Advanced Science, EarlyView.
This study identifies that RBM10 represses the transcriptional activity of RORB in liquid condensates, resulting in down‐regulation of NR1D1 and RIOK3 that further activates NF‐κB activity to facilitate lysosomal biogenesis and aggressive features of NB cells.
Yanhua Guo, Xiaojing Wang, Chunhui Yang, Zhijie Wang, Xiaolin Wang, Xinyue Li, Jiaying Qu, Shunchen Zhou, Liduan Zheng, Qiangsong Tong   +9 more
wiley   +1 more source

Homologous recombination between transferred and chromosomal immunoglobulin kappa genes. [PDF]

, 1988
Mark D. Baker, Marc J. Shulman
openalex   +1 more source

Serine/Threonine Kinase 33 as a Novel Target of Bufalin in Treatment of Triple‐Negative Breast Cancer

Advanced Science, EarlyView.
This study identified that STK33 may serve as a target of Bufalin, which induces the degradation of STK33 and inhibits the growth of TNBC. Furthermore, STK33 is highly expressed in TNBC and enhances TNBC cell proliferation by phosphorylating and stabilizing CCAR1.
Shilong Jiang, Junyan Liu, Hui Li, Chan Zou, Xiaoya Wan, Rong Gong, Ting Jiang, Changxin Zhong, Zonglin Chen, Zewu Zhu, Dongsheng Cao, Yan Cheng   +11 more
wiley   +1 more source