Results 341 to 350 of about 773,827 (377)

Hotspots of homologous recombination

Experientia, 1994
Homologous recombination occurs at higher than average frequency at and near hotspots. Hotspots are special nucleotide sequences recognized by proteins that promote, directly or indirectly, a rate limiting step of recombination. This review focuses on two well-studied examples, the Chi sites of the bacterium Escherichia coli and the M26 site of the ...
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Homologous recombination in plants

Experientia, 1994
In plants three different approaches have been used to study homologous DNA recombination; extrachromosomal recombination (ECR) between transfected DNA molecules, intrachromosomal recombination (ICR) between repeated genes integrated into and resident at the genome and recombination between introduced DNA and homologous sequences in the genome (gene ...
Puchta, H., Swoboda, P., Hohn, B.
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Mechanism of Eukaryotic Homologous Recombination

Annual Review of Biochemistry, 2008
Homologous recombination (HR) serves to eliminate deleterious lesions, such as double-stranded breaks and interstrand crosslinks, from chromosomes. HR is also critical for the preservation of replication forks, for telomere maintenance, and chromosome segregation in meiosis I.
Hannah L. Klein, Patrick Sung, Joseph San Filippo   +2 more
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Role of SIRT1 in homologous recombination

DNA Repair, 2010
The class III histone deacetylase (HDAC) SIRT1 plays a role in the metabolism, aging, and carcinogenesis of organisms and regulates senescence and apoptosis in cells. Recent reports revealed that SIRT1 also deacetylates several DNA double-strand break (DSB) repair proteins. However, its exact functions in DNA repair remained elusive. Using nuclear foci
Andreea Csernok, R. Kreienberg, Miriam Elisabeth Uhl, Sevtap Aydin, Susanne A. Gatz, Lisa Wiesmüller   +5 more
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Role of homologous recombination in carcinogenesis

Experimental and Molecular Pathology, 2003
Cancer develops when cells no longer follow their normal pattern of controlled growth. In the absence or disregard of such regulation, resulting from changes in their genetic makeup, these errant cells acquire a growth advantage, expanding into precancerous clones.
Robert H. Schiestl, Alexander J.R. Bishop   +1 more
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Homologous recombination in a mammalian plasmid

Molecular and General Genetics MGG, 1990
Bovine papillomavirus (BPV) shuttle vectors replicate as a circular plasmid in mouse cell nuclei without impairing host cell viability. We used these vectors to analyze homologous recombination in mammalian cells. When several BPV-based plasmids carrying direct repeats were introduced into C127 cells, we detected many recombinant plasmid molecules that
Ichizo Kobayashi, Yoshihiro Kitamura, Hiroshi Yoshikura   +2 more
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Metabolic regulation of homologous recombination repair by MRE11 lactylation

Cell, 2023
Yuping Chen, Jinhuan Wu, Linhui Zhai, Tingting Zhang, Hui Yin, Huanyao Gao, Fei Zhao, Zhe Wang, Xiaoning Yang, Mingpeng Jin, Bingsong Huang, Xin Ding, Rui Li, Jie Yang, Yiming He, Qianwen Wang, Weibin Wang, Jake A. Kloeber, Yunxuan Li, Bingbing Hao, Yuanyuan Zhang, Jiadong Wang, Minjia Tan, Ke Li, Ping Wang, Zhenkun Lou, Jian Yuan   +26 more
semanticscholar   +1 more source

Quality control of homologous recombination

Cellular and Molecular Life Sciences, 2014
Exogenous and endogenous genotoxic agents, such as ionizing radiation and numerous chemical agents, cause DNA double-strand breaks (DSBs), which are highly toxic and lead to genomic instability or tumorigenesis if not repaired accurately and efficiently.
Jun Huang, Ting Liu
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Methods for Homologous Recombination in Drosophila [PDF]

, 2008
We present detailed protocols for two methods of gene targeting in Drosophila. The first, ends-out targeting, is identical in concept to gene replacement techniques used routinely in mammalian and yeast cells. In Drosophila, the targeted gene is replaced by the marker gene white + (although options exist to generate unmarked targeted alleles).
Kent G. Golic, Wei J. Gong, Keith A. Maggert   +2 more
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Biochemistry of eukaryotic homologous recombination

2006
The biochemistry of eukaryotic homologous recombination caught fire with the discovery that Rad51 is the eukaryotic homolog of the bacterial RecA and T4 UvsX proteins; and this field is still hot. The core reaction of homologous recombination, homology search and DNA strand invasion, along with the proteins catalyzing it, are conserved throughout ...
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