Results 81 to 90 of about 166,356,720 (268)
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Journal of Governance and Public Policy, 2018 Based on the problem of research, the aim of this research are to determine and to asses deeply about monitoring function of Regional House of Representatives (DPRD) toward implementation of regional budget (APBD) regulation in Bulungan Regency in the ...
Iskandar Iskandar, Jimmy Nasroen
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Political Limits to the Processing of Policy Problems
Politics and Governance, 2013 This contribution addresses political limits to the processing of policy problems in the United States. Our foci are the forces that limit policymakers' attention to different aspects of problems and how this affects the prospects for problem resolution.
Peter J. May +2 more
doaj +1 more source
FEBS Open Bio, EarlyView.The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti +5 more
wiley +1 more source
Constitutional Judges: What Powers Does the House of Representatives Have?
Jurnal Media HukumThe House of Representatives did not extend the term of constitutional judge Aswanto on September 29, 2022, because Aswanto regularly nullified laws that they passed.
Septi Nur Wijayanti +4 more
doaj +1 more source
BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells
FEBS Open Bio, EarlyView.BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley +1 more source