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Process Steps for HPTLC Analysis

2023
This chapter briefly discusses the general aspects that can affect the result of TLC. Sample preparation, application of samples, preparation of development solvents, the steps involved in the development of TLC, visualization and derivatization, and detection of analyte are the key steps of chromatographic development.
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HPTLC and electrochromatography of rapeseed peptides

Food / Nahrung, 1992
AbstractPeptides belong to the little investigated nitrogen compounds of rapeseeds. Finlayson [2] reports that their content in rapeseed flour is ab. 2% including free aminoacids. According to Schwenke et al. [5] 13% of rapeseed nitrogen compounds are peptides and aminoacids.
R. Amarowicz, H. Kmita‐Glaźewska
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Experimental Aspects and Implementation of HPTLC

2010
High-Performance Thin-Layer Chromatography (HPTLC) is a sophisticated instrumentation technique. It has been reported in many publications to provide excellent separation and qualitative and quantitative analysis of a wide range of compounds, such as herbal and botanical dietary supplements, nutraceuticals, traditional western medicines, traditional ...
Rashmin B. Patel   +2 more
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On the problem of the purity of solvents in HPTLC

Chromatographia, 1985
The influence of stabilizers (2,6-di-tert-butyl-4-methylphenol and hydroquinone) and impurities in solvents on the reproducibility of the quantitative stability-indicating determination of mebeverine hydrochloride by means of high-performance thin-layer chromatography is reported.
J. A. De Schutter   +3 more
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Standardized HPTLC for Reproducible Chemical Fingerprinting

High-performance thin-layer chromatography (HPTLC) plays a crucial role in establishing the chemical fingerprint of natural products (NPs) during analysis. This technique involves standardized procedures for each step, ensuring the reproducibility of results.
Thi Kieu Tiên Do   +2 more
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HPTLC Determination of Atorvastatin in Plasma

Chromatographia, 2007
High performance liquid chromatography has been the most popular choice for the determination of atorvastatin. In this study, two-step isocratic chromatography on silica gel 60F254 HPTLC layer and densitometric quantitation at λ = 280 nm was developed for the separation of atorvastatin from plasma constituencies and sodium diclofenac as peak-tracer ...
A. Jamshidi, A. R. Nateghi
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Chemometrie in der HPTLC

2014
Chemometrie beschreibt die Anwendung mathematisch‐statistischer Methoden auf analytische Daten. Das Ziel dabei ist nicht nur, so viel Informationen wie moglich aus den Messdaten abzuleiten. Chemometrische Methoden helfen auch bei der Auswahl der optimalen Messparameter.
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HPTLC determination of tryptophanase

Journal of Pharmaceutical and Biomedical Analysis, 2000
T N, Vasudevan, V P, Kulkarni
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Quantitative Auswertung in der HPTLC

2014
Ziel einer quantitativen Messung ist die Gehaltsbestimmung des Analyten. Die Messwerte mussen dazu in Gehaltswerte umgerechnet werden. Die Umrechnung gelingt, wenn die mathematische Beziehung zwischen Mess- und Gehaltswerten bekannt ist. Dieser funktionelle Zusammenhang wird durch eine Kalibrierung bestimmt.
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