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Human bone cellsin vitro

Calcified Tissue International, 1985
Human bone cell cultures were established by maintaining collagenase-treated bone fragments in low Ca++ medium. The resulting cell cultures exhibited a high level of alkaline phosphatase activity and produced a significant increase in intracellular cAMP when exposed to the 1-34 fragment of human parathyroid hormone.
Pamela Gehron, Robey, John D, Termine
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Bone Regeneration by Grafting of Cultured Human Bone

Tissue Engineering, 2004
A 3-mL sample of bone marrow was collected from the iliac bones of 27 orthopedic patients (8 men and 19 women with a mean age of 56.1 years [range, 17 to 76 years]), followed by culture in standard culture medium (minimal essential medium containing 15% fetal bovine serum).
Takafumi, Yoshikawa   +3 more
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Chemical composition of human bone

Archives of Oral Biology, 1983
The mineral and non-collagenous organic components of normal human femoral cortex were examined following powdering, demineralization with EDTA and digestion with bacterial collagenase. The protein, hexose, sialic acid and uronic acid contents of the matrix were determined.
K J, Quelch   +3 more
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Testosterone metabolism in human bone

Acta Endocrinologica, 1980
Abstract. The conversion of [1,2,6,7-3H]testosterone2 to [3H]dihydrotestosterone has been assessed in ground spongiosa of normal and osteoporotic bone. The tissue was obtained from 23 patients, 18 women, 3 men and 2 children who were undergoing orthopedic surgery. The formation of dihydrotestosterone was demonstrated in all samples examined.
H U, Schweikert   +5 more
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Tympanometry in Human Temporal Bones

Archives of Otolaryngology - Head and Neck Surgery, 1970
Tympanometry is a technique used to assess the mobility and condition of the tympanic membrane and middle ear during variation of air pressure in the ear canal. The technique was applied to a series of 21 fresh human temporal bones, 11 of which were judged to be normal.
J L, Peterson, G, Lidén
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