Results 1 to 10 of about 19,354 (212)

Two Clonal Cell Lines of Immortalized Human Corneal Endothelial Cells Show either Differentiated or Precursor Cell Characteristics [PDF]

Cells Tissues Organs, 2008
Access to primary human corneal endothelial cells (HCEC) is limited and donor-derived differences between cultures exacerbate the issue of data reproducibility, whereas cell lines can provide sufficient numbers of homogenous cells for multiple experiments. An immortalized HCEC population was adapted to serum-free culture medium and repeated cloning was
Valtink, Monika, Gruschwitz, Rita, Funk, Richard H. W., Engelmann, Katrin   +3 more
semanticscholar   +6 more sources

Corneal Endothelial Tissue Bioengineering Using Cultured Human Corneal Endothelial Precursor Cells

, 2013
The cornea is composed of a multilayered epithelium, Bowman's membrane, stroma, Descemet's membrane, and endothelium. The corneal endothelium (CE) is a single layer of hexagonal cells that separates the corneal stroma from the aqueous humor of the anterior chamber.
Mimura, Tatsuya, Yokoo, Seiichi, Yamagami, Satoru   +2 more
semanticscholar   +5 more sources

Successful transportation of human corneal endothelial tissues without cool preservation in varying Indian tropical climatic conditions and in vitro cell expansion using a novel polymer

Indian Journal of Ophthalmology, 2014
Background: Though the transplantation of human corneal endothelial tissue (CET) separated from cadaver cornea is in practice, its transportation has not been reported.
Srinivas K Rao, John Sudhakar, Periyasamy Parikumar, Sundaram Natarajan, Aditya Insaan, Hiroshi Yoshioka, Yuichi Mori, Shigeo Tsukahara, Subramani Baskar, Sadananda Rao Manjunath, Rajappa Senthilkumar, Paramasivam Thamaraikannan, Thangavelu Srinivasan, Senthilkumar Preethy, Samuel J K Abraham   +14 more
doaj   +2 more sources

Human Corneal Endothelial Cell Precursors Isolated by Sphere-Forming Assay

Investigative Opthalmology & Visual Science, 2005
To isolate precursors of human corneal endothelial cells (HCECs) in vitro.HCECs were subjected to a sphere-forming assay in which spheres floated in serum-free medium containing growth factors. To promote differentiation, the isolated sphere colonies were plated in dishes coated with poly-L-lysine (PLL)/laminin or fetal bovine endothelium extracellular
Seiichi, Yokoo, Satoru, Yamagami, Yasuo, Yanagi, Saiko, Uchida, Tatsuya, Mimura, Tomohiko, Usui, Shiro, Amano   +6 more
openaire   +3 more sources

Progressive Insights into 3D Bioprinting for Corneal Tissue Restoration. [PDF]

Adv Healthc Mater
This review explores the potential of 3D bioprinting to replicate the complex structure and function of the human cornea. It highlights key advances in bioink development, printing modalities, and in vivo performance, while addressing current challenges and emerging strategies. The review emphasizes bioprinting's promise to overcome donor shortages and
Namli I, Gupta D, Singh YP, Datta P, Rizwan M, Baykara M, Ozbolat IT.   +6 more
europepmc   +2 more sources

Advances in the Role of Adipose Tissue in Promoting Injury Repair and Resist Infection [PDF]

Immun Inflamm Dis
ABSTRACT Background In recent years, adipose tissue (AT) transplantation has increasingly been noticed by many people in the field of tissue repair and regeneration. Accumulating evidence demonstrates that AT exerts dual functions in promoting tissue repair and conferring anti‐infective properties, with distinct biological effects attributed to its ...
Duan X, Li R, Fu L, Yang J, Zhan Z.
europepmc   +2 more sources

Applications of Multifunctional Hydrogel in Tissue Engineering and Regenerative Medicine [PDF]

MedComm (2020)
Schematic illustration showing multifunctional hydrogels in the application of tissue engineering and regenerative medicine. ABSTRACT Hydrogels, with excellent hydrophilicity and high‐water content, have emerged as highly versatile biomaterials for tissue engineering and regenerative medicine.
Lyu J, Liu X, Yang Q, Zhang Y, Wang X.
europepmc   +2 more sources

Effects of Rho-Associated Kinase (Rock) Inhibitors (Alternative to Y-27632) on Primary Human Corneal Endothelial Cells

Cells, 2023
(1) Rho-associated coiled-coil protein kinase (ROCK) signaling cascade impacts a wide array of cellular events. For cellular therapeutics, scalable expansion of primary human corneal endothelial cells (CECs) is crucial, and the inhibition of ROCK ...
Gary S. L. Peh, Francisco Bandeira, Dawn J H Neo, K. Adnan, Y. Hartono, H. Ong, S. Naso, Anandalakshmi Venkatraman, J. A. Gomes, Viridiana Kocaba, Jodhbir S. Mehta   +10 more
semanticscholar   +1 more source

Effect of Magnetic Microparticles on Cultivated Human Corneal Endothelial Cells

Translational Vision Science & Technology, 2023
Purpose To investigate effects of magnetic microparticles on movement of magnet controlled human corneal endothelial cells (HCECs). Methods Immortalized HCEC line (B4G12) and primary culture of HCECs were exposed to two commercially available magnetic ...
J. Park, Kangmin Lee, Choul-Yong Park
semanticscholar   +1 more source

A single-cell RNA-seq analysis unravels the heterogeneity of primary cultured human corneal endothelial cells

bioRxiv, 2023
The cornea is a transparent and avascular tissue located in front of the eye. Its inner surface is lined by a monolayer of corneal endothelial cells (CECs), which maintain the cornea transparency.
P. Català, Nathalie Groen, V. LaPointe, M. Dickman   +3 more
semanticscholar   +1 more source