Distribution of Precursors in Human Corneal Stromal Cells and Endothelial Cells
Purpose: We identified original tissue-committed precursors with limited self-renewal capacity from human corneal stromal (HCS) cells and human corneal endothelial (HCE) cells, then tried to determine the distribution and proliferative capacity of the precursors. Design: Experimental study.
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Human corneal endothelial cell precursors isolated by sphere-forming assay
PURPOSE. To isolate precursors of human corneal endothelial cells (HCECs) in vitro. METHODS. HCECs were subjected to a sphere-forming assay in which spheres floated in serum-free medium containing growth factors. To promote differentiation, the isolated sphere colonies were plated in dishes coated with poly-L-lysine (PLL)/laminin or fetal bovine ...
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Advancements in CRISPR-based therapies for ocular pathologies: from disease mechanisms to intervention strategies. [PDF]
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Mao Y +6 more
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Schadwinkel HM +10 more
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