Results 11 to 20 of about 93,317 (199)

Dasabuvir Inhibits Human Norovirus Infection in Human Intestinal Enteroids. [PDF]

mSphere, 2021
Although there is an urgent need to develop effective antiviral therapy directed against HuNoV infection, compound screening to identify anti-HuNoV drug candidates has not been reported so far.
Hayashi T, Murakami K, Hirano J, Fujii Y, Yamaoka Y, Ohashi H, Watashi K, Estes MK, Muramatsu M.   +8 more
europepmc   +9 more sources

EPIREGULIN creates a developmental niche for spatially organized human intestinal enteroids. [PDF]

JCI Insight, 2023
Epithelial organoids derived from intestinal tissue, called enteroids, recapitulate many aspects of the organ in vitro and can be used for biological discovery, personalized medicine, and drug development.
Childs CJ, Holloway EM, Sweet CW, Tsai YH, Wu A, Vallie A, Eiken MK, Capeling MM, Zwick RK, Palikuqi B, Trentesaux C, Wu JH, Pellón-Cardenas O, Zhang CJ, Glass I, Loebel C, Yu Q, Camp JG, Sexton JZ, Klein OD, Verzi MP, Spence JR.   +21 more
europepmc   +8 more sources

Replication of Human Norovirus in Human Intestinal Enteroids Is Affected by Fecal Sample Processing. [PDF]

Viruses
Human intestinal enteroids (HIEs) culture is an emerging model for assessing the infectivity of human noroviruses (HuNoVs). The model is based on detecting an increase in HuNoV RNA post-infection of HIEs.
Narwankar R, Esseili MA.
europepmc   +6 more sources

Novel Segment- and Host-Specific Patterns of Enteroaggregative Escherichia coli Adherence to Human Intestinal Enteroids. [PDF]

mBio, 2018
Enteroaggregative Escherichia coli (EAEC) is an important diarrheal pathogen and a cause of both acute and chronic diarrhea. It is a common cause of pediatric bacterial diarrhea in developing countries.
Rajan A, Vela L, Zeng XL, Yu X, Shroyer N, Blutt SE, Poole NM, Carlin LG, Nataro JP, Estes MK, Okhuysen PC, Maresso AW.   +11 more
europepmc   +6 more sources

New Insights and Enhanced Human Norovirus Cultivation in Human Intestinal Enteroids [PDF]

mSphere, 2020
Human noroviruses (HuNoVs) are highly contagious and cause acute and sporadic diarrheal illness in all age groups. In addition, chronic infections occur in immunocompromised cancer and transplant patients.
Khalil Ettayebi, Victoria R. Tenge, Nicolás Cortés-Penfield, Sue E. Crawford, Frederick H. Neill, Xi‐Lei Zeng, Xiaomin Yu, B. Vijayalakshmi Ayyar, Douglas G. Burrin, Sasirekha Ramani, Robert L. Atmar, Mary K. Estes   +11 more
semanticscholar   +7 more sources

Human Sapovirus Replication in Human Intestinal Enteroids. [PDF]

J Virol, 2023
Human sapoviruses (HuSaVs) are a frequent but overlooked cause of acute gastroenteritis, especially in children. Little is known about this pathogen, whose successful in vitro cultivation was reported only recently, in a cancer cell-derived line ...
Euller-Nicolas G, Le Mennec C, Schaeffer J, Zeng XL, Ettayebi K, Atmar RL, Le Guyader FS, Estes MK, Desdouits M.   +8 more
europepmc   +6 more sources

Human Intestinal Enteroids Model MHC-II in the Gut Epithelium. [PDF]

Front Immunol, 2019
The role of intestinal epithelial cells (IECs) in mucosal tolerance and immunity remains poorly understood. We present a method for inducing MHC class II (MHC-II) in human enteroids, "mini-guts" derived from small intestinal crypt stem cells, and show that the intracellular MHC-II peptide-pathway is intact and functional in IECs.
Wosen JE, Ilstad-Minnihan A, Co JY, Jiang W, Mukhopadhyay D, Fernandez-Becker NQ, Kuo CJ, Amieva MR, Mellins ED.   +8 more
europepmc   +7 more sources

Human Norovirus Replication in Human Intestinal Enteroids as Model to Evaluate Virus Inactivation. [PDF]

Emerg Infect Dis, 2018
Human noroviruses are a leading cause of epidemic and endemic acute gastroenteritis worldwide and a leading cause of foodborne illness in the United States.
Costantini V, Morantz EK, Browne H, Ettayebi K, Zeng XL, Atmar RL, Estes MK, Vinjé J.   +7 more
europepmc   +7 more sources

Optimizing Human Intestinal Enteroids for Environmental Monitoring of Human Norovirus. [PDF]

Food Environ Virol, 2021
Human noroviruses (HuNoV) are the leading cause of gastrointestinal illness and environmental monitoring is crucial to prevent HuNoV outbreaks. The recent development of a HuNoV cell culture assay in human intestinal enteroids (HIEs) has enabled detection of infectious HuNoV.
Overbey KN, Zachos NC, Coulter C, Schwab KJ.   +3 more
europepmc   +6 more sources

Replication in Human Intestinal Enteroids of Infectious Norovirus from Vomit Samples. [PDF]

Emerg Infect Dis, 2021
A typical clinical symptom of human norovirus infection is projectile vomiting. Although norovirus RNA and viral particles have been detected in vomitus, infectivity has not yet been reported. We detected replication-competent norovirus in 25% of vomit samples with a 13-fold to 714-fold increase in genomic equivalents, confirming infectious norovirus.
Hagbom M, Lin J, Falkeborn T, Serrander L, Albert J, Nordgren J, Sharma S.   +6 more
europepmc   +8 more sources