Results 11 to 20 of about 90,205 (94)
Advanced Science, Volume 12, Issue 9, March 6, 2025.In this work, a human primary cell‐based immunocompetent model is established to investigate early responses to STm infection. Intestinal fibroblasts and monocyte‐derived macrophages are embedded in decellularized small intestinal submucosa (SIS) from domestic pigs, followed by the development of an enteroid monolayer.
Spyridon Damigos +10 more
wiley +1 more source
Apical‐out Tubuloids for Accurate Kidney Toxicity Studies
Aggregate, Volume 6, Issue 3, March 2025.Apical‐out tubuloids are developed to study apical drug transport and drug‐induced nephrotoxicity. These tubuloids exhibit primary cilia and relevant structural proteins, indicating apical‐out polarity and enhanced functionality. Functional assays, including bovine serum albumin uptake, confirm their physiological relevance for studying apical ...
Yugyeong Lee +10 more
wiley +1 more source
Establishment of a Novel Caco‐2‐Based Cell Culture System for Human Sapovirus Propagation
Genes to Cells, Volume 30, Issue 2, March 2025.We identified that both Caco‐2 cells and in‐house Caco‐2/Cas9 cells are susceptible to human sapovirus (HuSaV) infection. By performing two rounds of single‐cell cloning from Caco‐2/Cas9 cells, we successfully established a highly susceptible cell line, designated as Caco‐2MC.
Yuya Fukuda +7 more
wiley +1 more source
Chemistry – A European Journal, Volume 31, Issue 10, February 17, 2025.We synthesized BODIPY dyes with benzyl ester and carboxylic acid groups, showing charge transfer, polarity‐dependent triplet state formation, and pH‐dependent emission. Fluorescence lifetime microscopy revealed distinct lifetime gradients in colon cancer cells and pig enteroids.
Aimee Sheehan +5 more
wiley +1 more source
Exploration, Volume 5, Issue 1, February 2025.We conducted a critical evaluation of decellularization techniques, quality control, cross‐linking, and modification methods for dECM scaffolds derived from different sources. The functional properties of dECM components as well as their recent applications in multi‐organ tissue engineering and biomedicine were thoroughly reviewed.
Juan Liu +9 more
wiley +1 more source
The Use of Gut Organoids: To Study the Physiology and Disease of the Gut Microbiota
Journal of Cellular and Molecular Medicine, Volume 29, Issue 4, February 2025.ABSTRACT The intestinal flora has attracted much attention in recent years. An imbalance in the intestinal flora can cause not only intestinal diseases but also cause a variety of parenteral diseases, such as endocrine diseases, nervous system diseases and cardiovascular diseases.
Ya Deng +13 more
wiley +1 more source
Mechanistic insight into human milk extracellular vesicle‐intestinal barrier interactions
Journal of Extracellular Biology, Volume 4, Issue 1, January 2025.Abstract Human milk extracellular vesicles (EVs) are crucial mother‐to‐baby messengers that transfer biological signals. These EVs are reported to survive digestion and transport across the intestine. The mechanisms of interaction between human milk EVs and the intestinal mucosa, including epithelial uptake remain unclear.
Xiang Luo +8 more
wiley +1 more source
Chemically Defined Organoid Culture System for Cholangiocyte Differentiation
Advanced Healthcare Materials, Volume 13, Issue 30, December 4, 2024.Cholangiocyte organoids are crucial for disease modeling and regenerative medicine but face limitations with animal‐derived hydrogels and limited function. This study presents a synthetic polyisocyanopeptide hydrogel combined with an optimized medium to enhance cholangiocyte organoid maturation. The resulting organoids show improved functional markers,
Zhenguo Wang +5 more
wiley +1 more source
Initial pig developmental stage influences intestinal organoid growth but not cellular composition
Animal Models and Experimental Medicine, Volume 7, Issue 6, Page 944-954, December 2024.We prepared organoids from jejunal and colonic specimens collected from pigs aged between 1 and 180 days to evaluate the impact of donor age on organoid growth, morphology and cellular composition. The age of the tissue donor affected organoid growth and morphology but did not influence its phenotype.
Camille Duchesne +6 more
wiley +1 more source
Intestinal Microecology in Pediatric Surgery-Related Gastrointestinal Diseases Current Insights and Future Perspectives [PDF]
arXiv, 2023 Intestinal microecology is established from birth and is constantly changing until homeostasis is reached. Intestinal microecology is involved in the immune inflammatory response of the intestine and regulates the intestinal barrier function. The imbalance of intestinal microecology is closely related to the occurrence and development of digestive ...
arxiv