Results 21 to 30 of about 93,317 (199)

Use of Human Intestinal Enteroids for Recovery of Infectious Human Norovirus from Berries and Lettuce. [PDF]

Foods, 2023
Norovirus (NoV) is the leading cause of viral foodborne gastroenteritis globally. Currently, the gold standard for detecting NoV in clinical, food, and environmental samples is via molecular-based methods, primarily RT-PCR. Nevertheless, there is a great
Wales SQ, Kulka M, Keinard B, Ngo D, Papafragkou E.   +4 more
europepmc   +5 more sources

Genetic Manipulation of Human Intestinal Enteroids Demonstrates the Necessity of a Functional Fucosyltransferase 2 Gene for Secretor-Dependent Human Norovirus Infection. [PDF]

mBio, 2020
Several studies have demonstrated that secretor status is associated with susceptibility to human norovirus (HuNoV) infection; however, previous reports found that FUT2 expression is not sufficient to allow infection with HuNoV in a variety of continuous
Haga K, Ettayebi K, Tenge VR, Karandikar UC, Lewis MA, Lin SC, Neill FH, Ayyar BV, Zeng XL, Larson G, Ramani S, Atmar RL, Estes MK.   +12 more
europepmc   +4 more sources

Human intestinal enteroids as a model of Clostridioides difficile-induced enteritis. [PDF]

Am J Physiol Gastrointest Liver Physiol, 2020
Clostridioides difficile is an important nosocomial pathogen that produces toxins to cause life-threatening diarrhea and colitis. Toxins bind to epithelial receptors and promote the collapse of the actin cytoskeleton. C.
Engevik MA, Danhof HA, Chang-Graham AL, Spinler JK, Engevik KA, Herrmann B, Endres BT, Garey KW, Hyser JM, Britton RA, Versalovic J.   +10 more
europepmc   +4 more sources

Bile acids and ceramide overcome the entry restriction for GII.3 human norovirus replication in human intestinal enteroids. [PDF]

Proc Natl Acad Sci U S A, 2020
Significance Many viral pathogens replicate in the human small intestine where they must invade the epithelial barrier that has evolved to protect the host against microbial assaults. Using a human norovirus strain that requires bile to replicate in stem
Murakami K, Tenge VR, Karandikar UC, Lin SC, Ramani S, Ettayebi K, Crawford SE, Zeng XL, Neill FH, Ayyar BV, Katayama K, Graham DY, Bieberich E, Atmar RL, Estes MK.   +14 more
europepmc   +4 more sources

Human norovirus exhibits strain-specific sensitivity to host interferon pathways in human intestinal enteroids. [PDF]

Proc Natl Acad Sci U S A, 2020
Significance Host innate immune responses are the first line of defense against viral infections, with the outcome of infection determined by the interplay between virus and host defense mechanisms.
Lin SC, Qu L, Ettayebi K, Crawford SE, Blutt SE, Robertson MJ, Zeng XL, Tenge VR, Ayyar BV, Karandikar UC, Yu X, Coarfa C, Atmar RL, Ramani S, Estes MK.   +14 more
europepmc   +5 more sources

Proliferative Cell Targeting and Epithelial Cell Turnover Fuels Hepatitis E Virus Replication in Human Intestinal Enteroids. [PDF]

Gastro Hep Adv
Santos-Ferreira N, Zhang X, Corneillie L, Van Dycke J, Chiu W, Montpellier C, Neyts J, Cocquerel L, Kaptein SJF, Rocha-Pereira J.   +9 more
europepmc   +3 more sources

Astrovirus replication in human intestinal enteroids reveals multi-cellular tropism and an intricate host innate immune landscape. [PDF]

PLoS Pathog, 2019
Human astroviruses (HAstV) are understudied positive-strand RNA viruses that cause gastroenteritis mostly in children and the elderly. Three clades of astroviruses, classic, MLB-type and VA-type have been reported in humans.
Kolawole AO, Mirabelli C, Hill DR, Svoboda SA, Janowski AB, Passalacqua KD, Rodriguez BN, Dame MK, Freiden P, Berger RP, Vu DL, Hosmillo M, O'Riordan MXD, Schultz-Cherry S, Guix S, Spence JR, Wang D, Wobus CE.   +17 more
europepmc   +4 more sources

Molecular Basis and Differentiation-Associated Alterations of Anion Secretion in Human Duodenal Enteroid Monolayers [PDF]

Frontiers in Cellular and Infection Microbiology, 2018
Background & Aims: Human enteroids present a novel tool to study human intestinal ion transport physiology and pathophysiology. The present study describes the contributions of Cl- and HCO3 - secretion to total cyclic adenosine monophosphate (cAMP ...
Avula, L.R. (Leela Rani), Donowitz, M. (Mark), Foulke-Abel, J. (Jennifer), Jonge, H.R. (Hugo) de, Singh, V. (Varsha), Tse, C.-M. (Chung-Ming), Yin, J. (Jianyi)   +6 more
core   +17 more sources

Standardization of an antiviral pipeline for human norovirus in human intestinal enteroids demonstrates nitazoxanide has no to weak antiviral activity. [PDF]

Antimicrob Agents Chemother, 2023
Human noroviruses (HuNoVs) are the leading cause of acute gastroenteritis. In immunocompetent hosts, symptoms usually resolve within 3 days; however, in immunocompromised persons, HuNoV infection can become persistent, debilitating, and sometimes life ...
Lewis MA, Cortés-Penfield NW, Ettayebi K, Patil K, Kaur G, Neill FH, Atmar RL, Ramani S, Estes MK.   +8 more
europepmc   +2 more sources

Human Intestinal Enteroids: New Models to Study Gastrointestinal Virus Infections. [PDF]

Methods Mol Biol, 2019
Human rotavirus (HRV) and human norovirus (HuNoV) infections are recognized as the most common causes of epidemic and sporadic cases of gastroenteritis worldwide. The study of these two human gastrointestinal viruses is important for understanding basic virus-host interactions and mechanisms of pathogenesis and to establish models to evaluate vaccines ...
Zou WY, Blutt SE, Crawford SE, Ettayebi K, Zeng XL, Saxena K, Ramani S, Karandikar UC, Zachos NC, Estes MK.   +9 more
europepmc   +6 more sources