Results 31 to 40 of about 93,317 (199)

Molnupiravir inhibits human norovirus and rotavirus replication in 3D human intestinal enteroids

Antiviral Research
Human norovirus (HuNoV) and human rotavirus (HRV) are the leading causes of gastrointestinal diarrhea. There are no approved antivirals and rotavirus vaccines are insufficient to cease HRV associated mortality. Furthermore, treatment of chronically infected immunocompromised patients is limited to off-label compassionate use of repurposed antivirals ...
Nânci Santos-Ferreira, Jana Van Dycke, Winston Chiu, Johan Neyts, Jelle Matthijnssens, Joana Rocha‐Pereira   +5 more
semanticscholar   +5 more sources

<i>Rhodococcus</i> spp. interacts with human norovirus in clinical samples and impairs its replication on human intestinal enteroids. [PDF]

Gut Microbes
Viral infections, particularly human norovirus (NoV), are a leading cause of diarrheal diseases globally. To better understand NoV susceptibility, it is crucial to investigate both host glycobiology and the influence of the microbiota.
Santiso-Bellón C, Randazzo W, Carmona-Vicente N, Peña-Gil N, Cárcamo-Calvo R, Lopez-Navarro S, Navarro-Lleó N, Yebra MJ, Monedero V, Buesa J, Gozalbo-Rovira R, Rodríguez-Díaz J.   +11 more
europepmc   +2 more sources

Use of Human Intestinal Enteroids to Detect 
Human Norovirus Infectivity. [PDF]

Emerg Infect Dis, 2019
Tools to detect human norovirus infectivity have been lacking. Using human intestinal enteroid cultures inoculated with GII.Pe-GII.4 Sydney-infected fecal samples, we determined that a real-time reverse transcription PCR cycle threshold cutoff of 30 may indicate infectious norovirus. This finding could be used to help guide infection control.
Chan MC, Cheung SKC, Mohammad KN, Chan JCM, Estes MK, Chan PKS.   +5 more
europepmc   +6 more sources

N-glycoproteomic analyses of human intestinal enteroids, varying in histo-blood group geno- and phenotypes, reveal a wide repertoire of fucosylated glycoproteins. [PDF]

Glycobiology
Human noroviruses, globally the main cause of viral gastroenteritis, show strain specific affinity for histo-blood group antigens (HBGA) and can successfully be propagated ex vivo in human intestinal enteroids (HIEs).
Nilsson J, Rimkute I, Sihlbom C, Tenge VR, Lin SC, Atmar RL, Estes MK, Larson G.   +7 more
europepmc   +2 more sources

Development of Functional Microfold (M) Cells from Intestinal Stem Cells in Primary Human Enteroids. [PDF]

, 2016
Background & aimsIntestinal microfold (M) cells are specialized epithelial cells that act as gatekeepers of luminal antigens in the intestinal tract.
Dunn, James CY, Eckmann, Lars, Hanson, Elaine M, Kobayashi, Masae, Lei, Nan Ye, Lewis, Michael, Martín, Martín G, Rouch, Joshua D, Scott, Andrew, Solorzano-Vargas, R Sergio, Stelzner, Matthias G, Wang, Jiafang   +11 more
core   +6 more sources

Intestinal Enteroid Culture for Human Astroviruses [PDF]

BIO-PROTOCOL, 2020
Human astroviruses (HAstV) are non-enveloped, positive-sense single stranded RNA viruses that typically cause gastroenteritis in children, the elderly and among immunocompromised individuals. Some HAstV species have also been implicated in neurological diseases.
Irene Owusu, Carmen Mirabelli, Abimbola Kolawole   +2 more
openaire   +4 more sources

Experimental Modeling of Necrotizing Enterocolitis in Human Infant Intestinal Enteroids

Journal of Investigative Surgery, 2020
There are several limitations when using a single cell culture to recapitulate the findings in a complex organism and results often vary between species, when proxy animal models are studied.Human enteroids have allowed for study of human disease in complex multicellular culture systems. Here we present the novel use of human infant enteroids generated
Christie Buonpane, Guillermo Ares, Carrie Yuan, Camille Schlegel, Heather Liebe, Catherine J. Hunter   +5 more
openaire   +3 more sources

Intestinal Stem Cell-Derived Enteroids From Morbidly Obese Patients Preserve Obesity-Related Phenotypes: Elevated Glucose Absorption and Gluconeogenesis [PDF]

Molecular Metabolism, 2021
OBJECTIVE: The mechanisms behind the efficacy of bariatric surgery (BS) for treating obesity and type 2 diabetes, particularly with respect to the influence of the small bowel, remain poorly understood.
Baetz, Nicholas W, Blutt, Sarah E, Estes, Mary K, Fayad, Lea, Hasan, Nesrin M, Johnson, Kelli F, Kovbasnjuk, Olga, Kumbhari, Vivek, Sherman, Vadim, Yin, Jianyi, Zachos, Nicholas C   +10 more
core   +4 more sources

Recovery of Infectious Human Norovirus GII.4 Sydney From Fomites via Replication in Human Intestinal Enteroids. [PDF]

Front Cell Infect Microbiol, 2021
Contamination of fomites by human norovirus (HuNoV) can initiate and prolong outbreaks. Fomite swabbing is necessary to predict HuNoV exposure and target interventions. Historically, swab recovered HuNoV has been measured by molecular methods that detect
Overbey KN, Zachos NC, Coulter C, Jacangelo J, Schwab KJ.   +4 more
europepmc   +2 more sources

Overcoming host restrictions to enable continuous passaging of GII.3 human norovirus in human intestinal enteroids [PDF]

Sci Adv
The establishment of human intestinal enteroids (HIEs) as a model for human norovirus (HuNoV) replication has been transformative for studying this leading cause of gastroenteritis.
Kaur G, Crawford S, Javornik Cregeen S, Surathu A, Ayyar B, Apostol C, Phuc H, Ettayebi K, Boussattach A, Zeng X, Blutt S, Doddapaneni H, Muzny D, Coarfa C, Anish R, Prasad B, Atmar R, Ramani S, Estes M.   +18 more
europepmc   +2 more sources