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Pyruvate kinase from human skeletal muscle

Molecular and Cellular Biochemistry, 1975
A simple method is described for the isolation of crystalline pyruvate kinase from human skeletal muscle. The enzyme was purified by ammonium sulfate fractionation, heat treatment and crystallization. Two crystal forms of pyruvate kinase differing in solubility but not in specific activity were found.
B, Baranowska, T, Baranowski
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Lysosomal storage in human skeletal muscle

Human Pathology, 1986
Skeletal muscle is involved symptomatically in two lysosomal storage diseases, acid maltase deficiency and a similar condition in which enzyme levels are normal. Asymptomatic storage in skeletal muscle cells is found in Batten-Kufs' disease (ceroid lipofuscinosis), Fabry's disease, and mannosidosis, as well as in rare patients with an unidentified ...
S, Carpenter, G, Karpati
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Glutamine transport in human skeletal muscle

American Journal of Physiology-Endocrinology and Metabolism, 1993
Sarcolemmal vesicles isolated from human skeletal muscle obtained at surgery showed approximately 14-fold enrichment of sarcolemmal marker enzymes 5'-nucleotidase and K-stimulated phosphatase. [3H]glutamine transport in these vesicles was stereospecific, largely Na dependent, and tolerated Li-for-Na substitution.
A, Ahmed   +3 more
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Human skeletal muscle ageing atlas

2022
AbstractSkeletal muscle ageing increases the incidence of age-associated frailty and sarcopenia in the elderly worldwide, leading to increased morbidity and mortality. However, our understanding of the cellular and molecular mechanisms of muscle ageing is still far from complete.
Veronika R. Kedlian   +26 more
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Ischemic Tolerance of Human Skeletal Muscle

Annals of Plastic Surgery, 1991
Until now, the ischemic tolerance of muscle tissue has not been adequately understood. Even when muscle vitality is lost, the perfusion matrix of the muscle flaps is retained. Because of toxic decomposition, however, irreversibly damaged muscle cells almost certainly increase the rate of complications.
P, Eckert, K, Schnackerz
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Parkin expression in human skeletal muscle

Journal of Clinical Neuroscience, 2005
Parkin is known to be present in human neurons and peripheral nerves. Using an antibody against parkin protein we have now demonstrated that parkin is also expressed in the sarcoplasm and sarcolemmal region of human skeletal muscle fibres. We have also found different age-related patterns of expression with increase in intensity and organization of ...
P, Serdaroglu   +3 more
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Human Skeletal Muscle‐on‐a‐Chip

The FASEB Journal, 2019
A comprehensive understanding of the emergent properties of in vitro skeletal muscle growth and development is still in its infancy. To address this gap in our current knowledge, we have developed a stretchable micropatterned 3D human skeletal muscle ...
A. Naik   +7 more
semanticscholar   +1 more source

Lipid autoxidation in human skeletal muscle

Clinica Chimica Acta, 1974
Abstract A standard method of measuring the susceptibility of human skeletal muscle to autoxidation, based on malonyldialdehyde generation during a 24-h incubation, was devised and the major variables influencing this process were studied. The rate of autoxidation in muscle is largely a function of the efficiency of inhibitory antioxidant mechanisms.
W, McMurray, T L, Dormandy
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Unusual mitochondria in human skeletal muscle

Experientia, 1975
Bei elektronenmikroskopischen Untersuchungen an Muskelbiopsien von Kranken mit progressiver Sklerodermie konnten in einem Fall ungewohnlich viele, grosstenteils kugelige Mitochondrien mit einem Durchmesser von 0,03–0,05 μm subsarkolemmal beobachtet werden. Zwischen diesen befanden sich zahlreiche stabchenformige Mitochodnrien mit einer Lange von 1–3 μm
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Isolation of sarcolemma from human skeletal muscle

Zeitschrift f�r Anatomie und Entwicklungsgeschichte, 1974
An isolation procedure for sarcolemma of human skeletal muscle is described. The method includes the possibility to prepare sarcoplasmic reticulum from the same muscle fibres. Electron microscopy reveals a homogeneous final fraction of 80–90% myofibre enveloping membranes contaminated by blood vessel membranes.
H, Engel, K, Gorgas
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