Results 111 to 120 of about 34,961,696 (347)

In situ hybridization: Methods and applications [PDF]

Journal of Clinical Laboratory Analysis, 1997
In situ hybridization (ISH) combines molecular biological techniques with histological and cytological analysis of gene expression. RNA and DNA can be readily localized in specific cells with this method. ISH has been useful as a research tool, and recent studies have used this technique in the diagnostic pathology laboratory and in microbiology for ...
L, Jin, R V, Lloyd
openaire   +2 more sources

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

A colorimetric method for DNA hybridization

Nucleic Acids Research, 1984
A general method has been developed which allows crosslinks to be produced between proteins and single-stranded DNA. Such single-stranded DNA protein complexes have been tested for blot hybridization using two colorimetrically detectable enzymes, namely peroxidase and alkaline phosphatase, as the protein moiety of the probe.
M, Renz, C, Kurz
openaire   +3 more sources

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source

Parallel and sequential hybrid methods for a finite family of asymptotically quasi $$\phi $$ϕ-nonexpansive mappings [PDF]

, 2015
In this paper we study some novel parallel and sequential hybrid methods for finding a common fixed point of a finite family of asymptotically quasi $$\phi $$ϕ-nonexpansive mappings.
Pham Ky Anh, D. Hieu
semanticscholar   +1 more source

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway

FEBS Open Bio, EarlyView.
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo, Sandy Richter, Christoph Bach, Wieland Kiess, Anna Kirstein, Diana Le Duc, Antje Garten   +6 more
wiley   +1 more source

Microwave Detection of Brain Injuries by Means of a Hybrid Imaging Method

IEEE Open Journal of Antennas and Propagation, 2020
Brain injuries represent a critical situation, where both detection and monitoring should be quick and accurate at the same time. Microwave techniques are thus gaining attention in the diagnostic process of these diseases.
Alessandro Fedeli, Claudio Estatico, Matteo Pastorino, Andrea Randazzo   +3 more
doaj   +1 more source

Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface

FEBS Open Bio, EarlyView.
HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana, Avigail Yariv, Tal Oppenheim, Hadar Amartely, Dana Reichmann, Oded Livnah   +5 more
wiley   +1 more source

APPLICATION OF HYBRID RANDOM SEARCH METHOD TO OPTIMISATION OF ENGINEERING SYSTEMS’ PARAMETERS

Научный вестник МГТУ ГА, 2018
This paper presents a modification of the Luus-Jaakola global optimization method, which belongs to the class of metaheuristic algorithms. A hybrid method is suggested, using a combination of random search methods: Luus-Jaakola method, adaptive random ...
A. V. Panteleev, D. A. Rodionova
doaj   +1 more source

Hybrid methods for ICD-10 coding of death certificates

Louhi@EMNLP, 2016
ICD-10 coding of death certificates has received renewed attention recently with the organization of the CLEF eHealth 2016 clinical information extraction task (CLEF eHealth 2016 Task 2).
Pierre Zweigenbaum, T. Lavergne
semanticscholar   +1 more source