Results 331 to 340 of about 1,909,880 (377)

In situ hybridization with oligonucleotide probes

2002
Publisher Summary In situ hybridization (ISH) is an important method for tracing the regional and cellular sites of gene expression (mRNA distribution) within a tissue. This method comes into its own when applied to the intermingled cell types of the brain.
William Wisden, Brian J. Morris
openaire   +3 more sources

Label-free DNA hybridization probe based on a conducting polymer.

Journal of the American Chemical Society, 2003
A new approach to the realization of the electrochemical DNA hybridization probe is described. It is based on the exchange of chloride ion between the polypyrrole layer and the buffer.
Liz Thompson, J. Kowalik, M. Josowicz, J. Janata   +3 more
semanticscholar   +1 more source

Detection of Hybridized Probe

Current Protocols in Molecular Biology, 1989
AbstractAutoradiography is used to detect and quantitate radioactive probe hybridized to cytological preparations. Autoradiographic film is used to detect 32P‐ or 35S‐labeled probe, and can be useful in experiments dealing with large organs or tissues. Emulsion autoradiography is required to obtain resolution at the level of a single cell.
openaire   +3 more sources

Effect of Probe–Probe Distance on the Stability of DNA Hybrids on Surfaces

Analytical Chemistry, 2017
We have used temperature gradient surface plasmon resonance (SPR) measurements to quantitatively evaluate how the stability of different types of hybrids formed with DNA probes on surfaces is affected by probe spacing. SPR sensors with different average surface densities of probes were prepared by coadsorbing probes with lateral spacers strands ...
Lucyano J. A. Macedo, Erin N. Miller, Aric Opdahl   +2 more
openaire   +3 more sources

Hybridization and detection of digoxigenin probes on RNA blots

Molecular Biotechnology, 1996
This article describes nonradioactive probing of a Northern blot. The method employs digoxigenin-labeled probes. Antidigoxigenin antibody/alkaline phosphate conjugate, and a chemiluminescent substrate are subsequently used in the detection system.
Elizabeth Davies, Rachel Hodge, Peter G. Isaac   +2 more
openaire   +5 more sources

Production and Use of Nonradioactive Hybridization Probes

2003
Molecular hybridization is a useful technique for identifying specific target sequences even when they are present as a single copy in a complex population. It can be performed either on a solid matrix on which pure DNA (or RNA) is bound (blot hybridization) or on tissue sections (in situ hybridization). Until recently, the probes used in hybridization
Victor T.-W. Chan, James O'd. McGee
openaire   +4 more sources

DNA hybridization probe for endoparasitism by Microplitis croceipes (Hymenoptera: Braconidae)

, 1998
We describe a DNA hybridization assay for an insect endoparasitoid. The probe is a digoxigenin-labeled 438 bp fragment from a genomic library of the braconid Microplitis croceipes (Cresson), a larval endoparasitoid of heliothine noctuids.
M. Greenstone, M. Edwards
semanticscholar   +1 more source

Quantitation of casein messenger ribonucleic acid sequences using a specific complementary DNA hybridization probe.

Biochemistry, 1976
Two highly purified rat casein mRNA fractions were used as templates to synthesize complementary DNA (cDNA) hybridization probes using RNA-directed DNA polymerase isolated from avian myeloblastosis virus.
J. Rosen, S. Barker
semanticscholar   +1 more source

Biotinylated Probes in Colony Hybridization

1998
Colony hybridization is a procedure that allows the detection of cells containing nucleic acid sequences of interest (1). In this method, microbial colonies grown on, or transferred to, a supporting membrane are lysed and their nucleic adds denatured to single strands and fixed in place on the membrane.
openaire   +5 more sources

Hybridization probe for femtomolar quantification of selected nucleic acid sequences on a disposable electrode.

Analytical Chemistry, 2006
Mixed monolayers of electroactive hybridization probes on gold surfaces of a disposable electrode were investigated as a technology for simple, sensitive, selective, and rapid gene identification.
D. Jenkins, B. Chami, M. Kreuzer, G. Presting, A. Alvarez, B. Liaw   +5 more
semanticscholar   +1 more source