Results 71 to 80 of about 17,663 (189)

In vitro activation of [FeFe] hydrogenase: new insights into hydrogenase maturation

JBIC Journal of Biological Inorganic Chemistry, 2007
The in vitro activation of the [FeFe] hydrogenase is accomplished by combining Escherichia coli cell extracts containing the heterologously expressed inactive HydA with extracts in which hydrogenase-specific maturation proteins HydE, HydF, and HydG are expressed in concert. Interestingly, the process of HydA activation occurs rapidly and in the absence
McGlynn, SE, Ruebush, SS, Naumov, A, Nagy, LE, Dubini, A, King, PW, Broderick, JB, Posewitz, MC, Peters, JW   +8 more
openaire   +4 more sources

Understanding the Catalytic Determinant role of Diaphorase‐Like Subunit in Formate Dehydrogenases via Redox Couples

Advanced Science, Volume 13, Issue 46, 17 August 2026.
A unique mechanism of catalytic bias regulated by diaphorase‐like subunit in formate dehydrogenase from Rhodobacter aestuarii is revealed. The diaphorase‐like subunit functions act as a biological “voltage rheostat” that controls the slow release of NADH to regulate redox balance, biasing the enzyme's catalytic preference toward CO2 reduction over ...
Kuncheng Zhang, Weisong Liu, Hao Su, Huijuan Cui, Yuanming Wang, Zhiguang Zhu, Chun You, Lingling Zhang   +7 more
wiley   +1 more source

Molecular and energetic basis of histidine switch dynamics in respiratory complex I

Protein Science, Volume 35, Issue 8, August 2026.
Abstract The respiratory complex I in mitochondria and bacteria drives the two‐electron reduction of quinone to pump protons across the membrane. The molecular basis of this catalytic reaction remains enigmatic despite significant progress in structural characterization of the complex.
Erik Endres, Mahdi Torabi, Mai Jousmäki, Kim Vy Huynh, Cristina Pecorilla, Oleksii Zdorevskyi, Volker Zickermann, Vivek Sharma   +7 more
wiley   +1 more source

Global Carbon Investment in Terrestrial Biological Nitrogen Fixation

Global Biogeochemical Cycles, Volume 40, Issue 8, August 2026.
Abstract Biological nitrogen (N) fixation (BNF) provides the N needed to produce proteins and other biological building blocks, helping feed humanity and mitigate climate change. Due to its high energetic cost compared to other forms of N acquisition, biotic investment in BNF indicates N limitation. Globally gridded BNF flux data provide an opportunity
Duncan N. L. Menge, Carla R. Reis Ely, Steven S. Perakis, Sian Kou‐Giesbrecht, Cory C. Cleveland, Sasha C. Reed, Benton N. Taylor, Sarah A. Batterman, Timothy E. Crews, Katherine A. Dynarski, Jennifer L. Funk, Maga Gei, Kevin L. Griffin, Michael J. Gundale, David F. Herridge, Sarah E. Jovan, Mark B. Peoples, Johannes Piipponen, Emilio Rodríguez‐Caballero, Verity G. Salmon, Fiona M. Soper, Anika P. Staccone, Bettina Weber, Amelia A. Wolf, Nina Wurzburger   +24 more
wiley   +1 more source

Metabolic Flux and Growth Profiling of Megasphaera cerevisiae for Medium‐Chain Fatty Acid Synthesis

Environmental Microbiology Reports, Volume 18, Issue 4, August 2026.
Characterising the metabolism of medium‐chain fatty acids in Megasphaera cerevisiae, based on genome‐scale metabolic modelling and integration with experimental data obtained from controlled bioreactor cultivations. The iterative approach aligns in silico predictions with empirical observations and a refined model was obtained.
Wael Sabra, Sonia Villotti, Joachim Fensterle, An‐Ping Zeng, Ahmed M. Haddad   +4 more
wiley   +1 more source

Delimiting the Function of the C-Terminal Extension of the Escherichia coli [NiFe]-Hydrogenase 2 Large Subunit Precursor

Frontiers in Microbiology, 2019
The active site of all [NiFe]-hydrogenases (Hyd) has a bimetallic NiFe(CN)2CO cofactor that requires the combined action of several maturation proteins for its biosynthesis and insertion into the precursor form of the large subunit of the enzyme ...
Constanze Pinske, Claudia Thomas, Kerstin Nutschan, R. Gary Sawers   +3 more
doaj   +1 more source

Advancing the Resolution of the Sulfur Compound Disproportionation Puzzle in Dissulfuribacter Thermophilus S69ᵀ Through Quantitative Label‐Free Comparative Proteomics

Environmental Microbiology Reports, Volume 18, Issue 4, August 2026.
To date, only three microorganisms have been functionally characterised for sulfur disproportionation. Our work identifies a fourth example and provides novel proteomic insights into its underlying pathways. These findings advance our understanding of microbial sulfur catabolism and highlight the ecological role of D.
Marilina Fernandez, Barbara Schoepp‐Cothenet, Marie Hemon, Carine Rodrigues‐Machado, Lukas V. F. Novák, Xavier Philippon, Sébastien Laurent, Céline Henry, Karine Alain   +8 more
wiley   +1 more source

Role of Nickel in Microbial Pathogenesis

Inorganics, 2019
Nickel is an essential cofactor for some pathogen virulence factors. Due to its low availability in hosts, pathogens must efficiently transport the metal and then balance its ready intracellular availability for enzyme maturation with metal toxicity ...
Robert J. Maier, Stéphane L. Benoit
doaj   +1 more source

Hydrogen Oxidation Influences Glycogen Accumulation in a Verrucomicrobial Methanotroph

Frontiers in Microbiology, 2019
Metabolic flexibility in aerobic methane oxidizing bacteria (methanotrophs) enhances cell growth and survival in instances where resources are variable or limiting.
Carlo R. Carere, Ben McDonald, Hanna A. Peach, Chris Greening, Daniel J. Gapes, Christophe Collet, Matthew B. Stott   +6 more
doaj   +1 more source

Hydrogenases and oxygen

Chemical Science, 2012
Hydrogenases represent a heterogeneous group of enzymes consisting of three evolutionary unrelated classes, i.e. [NiFe], [FeFe] and [Fe] hydrogenases. They allow the uni-cellular organisms in which they are expressed to use hydrogen as energy source or to reduce protons as a sink for excess reduction equivalents.
Stiebritz Martin Tillmann, Reiher Markus
openaire   +1 more source