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Photogeneration of hydroxyl radicals for footprinting
Analytical Biochemistry, 1992Hydroxyl radicals yield footprints of DNA-ligand interactions that are uniform in intensity and display single base pair resolution. It is shown here that brief illumination of dilute aqueous solutions of hydrogen peroxide with a standard uv transilluminator can be used to generate hydroxyl radicals for footprinting studies.
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Isotopic Spectra of the Hydroxyl Radical
The Journal of Physical Chemistry A, 2013Rotational spectra of OH and its isotopologues have been precisely measured using high efficiency terahertz (THz) sources. The measurements are compared with existing data and are useful for global modeling. For the first time, microwave measurements of the Λ-doubling transitions of the (17)OH isotopologue are combined with THz data successfully ...
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DNA Footprinting with the Hydroxyl Radical
Free Radical Research Communications, 1991The Fenton reaction of iron(II) EDTA with hydrogen peroxide, performed in the presence of ascorbate ion, has proven to be useful as a probe of structure in DNA systems. Two aspects of this chemistry are discussed: the identity of the active DNA cleaving agent produced by this reagent, and the application of the Fenton reaction to the determination of ...
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Hydroxyl Radicals Attack Metallic Gold
Angewandte Chemie, 2010Anna Maria, Nowicka +3 more
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Free-radical hydroxylation of steroids
Journal of the American Chemical Society, 1972A, Rotman, Y, Mazur
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