Results 31 to 40 of about 15,700 (254)

Monitoring Cl− Movement in Single Cells Exposed to Hypotonic Solution [PDF]

Journal of Membrane Biology, 2005
Self-referencing ion--selective electrodes (ISEs), made with Chloride Ionophore I-Cocktail A (Fluka), were positioned 1-3 microm from human embryonic kidney cells (tsA201a) and used to record chloride flux during a sustained hyposmotic challenge. The ISE response was close to Nernstian when comparing potentials (VN) measured in 100 and 10 mM NaCl ...
Garber, S.S., Messerli, M.A., Hubert, M., Lewis, R., Hammar, K., Indyk, E., Smith, P.J.S   +6 more
openaire   +3 more sources

MITF maintains genome stability in nonmelanocyte lineages

Molecular Oncology, EarlyView.
MITF is essential for melanocyte survival and acts as an oncogene in 10%–20% of melanomas. We show that MITF depletion causes genome instability in nonmelanocytic cells, leading to LATS2‐mediated P53 activation, cell cycle arrest, and apoptosis. This study highlights the role of MITF as a genome maintenance factor beyond the melanocyte lineage. Created
Drifa H. Gudmundsdottir, Adrián López García de Lomana, Thejus B. Venkatesh, Kritika Kirty, Snaevar Sigurdsson, Linda Vidarsdottir, Ramile Dilshat, Erla Sveinbjornsdottir, Snaedis Ragnarsdottir, Daniel H Magnusson, Maria R. Bustos, Eirikur Steingrimsson, Thorkell Gudjonsson, Stefan Sigurdsson   +13 more
wiley   +1 more source

Effect of pH and Hypo-Osmotic Stress on NaCl Electrolyte Permeation across Red Blood Cell Membrane

Journal of Applied Sciences and Environmental Management, 2019
The study compares the effects produced by H+ concentrations in hypo-osmotically stressed NaCl solutions on permeation through RBC membrane. Iso-osmotic solutions of NaCl (160 mM) were prepared taking into account their molecular weight and osmotic ...
B.O. Atolaiye, D.U. Edward-Ekpu, T.O. Bamidele   +2 more
doaj   +2 more sources

Effect of isotonic and hypotonic fluid therapy on serum sodium: A Randomized Controlled Trial

Acta Medica Iranica, 2020
There is little consensus about type of the maintenance fluid therapy and it’s effect on serum sodium in adults. Thus, this study was conducted to assess the effect of maintenance fluid therapy on serum sodium of hospitalized patients in intensive care ...
Moslem Sedaghattalab, Soheila Khajezadeh, Akvan Paymard, Parisa Mansuri, Rozina Abasi Larki, Leila Manzouri   +5 more
doaj   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

Hypotonic Stress Increases Efficacy of Rilmakalim, but Not Pinacidil, to Activate ATP-Sensitive K+ Current in Guinea Pig Ventricular Myocytes

Journal of Pharmacological Sciences, 2004
The aim of this study was to investigate the influence of hypotonic challenge on the effects of potassium channel openers (PCO), rilmakalim and pinacidil, on activation of the ATP-sensitive K+ current.
Ivan Kocić, Yuji Hirano, Masayasu Hiraoka   +2 more
doaj   +1 more source

Maintenance enteral electrolyte solutions for neonatal calves: sodium acetate and osmolarity effects [PDF]

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2020
The use of hypotonic electrolytic solutions in enteral fluid therapy is still understudied in calves. The objective of the present study was to evaluate the effects of maintenance enteral electrolytic solutions with different concentrations of sodium ...
A.P. Lima, J.D. Ribeiro Filho, P.A.N. Ermita, L.C.F. Baptista Filho, M.F.B. Avanza, R.B. Viana, P.V.M. Santos, M.O. Silva, L.C. Monteiro, W.M.F. Dantas   +9 more
doaj   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Effects of Hypotonic and Isotonic Enteral Electrolyte Solutions Administered in Continuous Flow in Weaned Foals

Frontiers in Veterinary Science, 2020
The effects of fluid therapy with maintenance enteral electrolytic solutions administered by nasogastric route in continuous flow have not previously been studied in weaned foals.
Lorena Chaves Monteiro, Rinaldo Batista Viana, Marcel Ferreira Bastos Avanza, Pedro Ancelmo Nunes Ermita, Caio Monteiro Costa, Samuel Rodrigues Alves, Paulo Vinícius de Morais Santos, Micheline Ozana da Silva, Daniel Atila de Barros Balbino, Felipe Sperandio de Mattos, Raffaella Bertoni Cavalcanti Teixeira, José Dantas Ribeiro Filho   +11 more
doaj   +1 more source

Developmental and Epileptic Encephalopathy due to Biallelic Pathogenic Variants in PIGM

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective PIGM encodes a critical enzyme in the glycosylphosphatidylinositol (GPI)‐anchor biosynthesis pathway. While promoter‐region mutations in PIGM have been associated with a relatively mild phenotype characterized by portal vein thrombosis and absence seizures, recent evidence suggests that coding‐region mutations result in a more severe
Júlia Sala‐Coromina, Anna Marcé‐Grau, Barbara Masotto, Marta Codina, Lamia BenJemaa, Yasmina Elaribi, Mónica Martinez‐Gallo, Roger Colobran, Angel Sanchez‐Montañez, Irene Valenzuela, Yoshiko Murakami, Alfons Macaya   +11 more
wiley   +1 more source