Results 141 to 150 of about 114,639 (262)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

The revised three-step detour pathway in dolichol biosynthesis is evolutionarily conserved in budding yeast. [PDF]

Proc Natl Acad Sci U S A
Hanaoka K, Matsunaga K, Shimizu S, Sakai S, Pichler H, Funato K.   +5 more
europepmc   +1 more source

De novo synthesis of short‐chain aldehydes and hydrocarbons secreted by the brown marmorated stink bug Halyomorpha halys

FEBS Open Bio, EarlyView.
The brown marmorated stink bug, Halyomorpha halys, mainly secretes 4‐oxo‐(E)‐2‐hexenal, (E)‐2‐decenal, dodecane and tridecane, a mixture representative of that released by many heteropterans. We demonstrated that H. halys can synthesise these secretory components de novo using dietary glucose.
Haruna Fujimori, Koji Noge
wiley   +1 more source

Editorial: Interdisciplinary approaches to enhancing child and adolescent mental health in schools. [PDF]

Front Child Adolesc Psychiatry
Ramos-Álvarez O, Arufe-Giráldez V, Ibáñez-García A.   +2 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Causal effects of breastfeeding promotion on child health: understanding the role of nutrition. [PDF]

Swiss J Econ Stat
Brenøe AA, Stearns J, Martin RM.
europepmc   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Direct Construction of Magnetic and Electrical Two-Dimensional Radical Covalent Organic Frameworks. [PDF]

Nat Commun
Paitandi RP, Giri M, Koyae R, Nobuoka M, Tsutsui Y, Shuku Y, Koo YH, Li Z, Sakurai Y, Suzuki K, López-Cabrelles J, Furukawa S, Kaji H, Suda M, Ghosh S, Seki S.   +15 more
europepmc   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source

AI-Enabled Digital Health Promotion and Prevention: Computational Literature Review. [PDF]

JMIR AI
Girão Carrilho M, Costa Pinto D, Wagner R, Rohden SF, Telo de Arriaga M, Quelhas Pinto L.   +5 more
europepmc   +1 more source