Microbiome profiling by Illumina sequencing of combinatorial sequence-tagged PCR products [PDF]
We developed a low-cost, high-throughput microbiome profiling method that uses combinatorial sequence tags attached to PCR primers that amplify the rRNA V6 region.
A Chao+47 more
core +14 more sources
Fluorescent amplification for next generation sequencing (FA-NGS) library preparation. [PDF]
BACKGROUND:Next generation sequencing (NGS) has become a universal practice in modern molecular biology. As the throughput of sequencing experiments increases, the preparation of conventional multiplexed libraries becomes more labor intensive ...
Chiniquy, Jennifer+3 more
core +1 more source
Molecular Genetic Analysis of the Autosomal Recessive Non-Syndromic Inherited Retinitis Pigmentosa
Introduction: 90% of visually impaired people live in developing countries. There are various types of vision impairment, but the focus of the current study is retinitis pigmentosa (RP). Up to now, 150 mutations have been reported that are linked with RP.
Faiza S M Habib+9 more
semanticscholar +1 more source
Cassava begomovirus species diversity changes during plant vegetative cycles
Cassava is a root crop important for global food security and the third biggest source of calories on the African continent. Cassava production is threatened by Cassava mosaic disease (CMD), which is caused by a complex of single-stranded DNA viruses ...
Anna E. Dye+18 more
semanticscholar +1 more source
Genome Features of Probiotic Bifidobacteria Determining Their Strain-Specific Properties
The aim of the study was to analyze the genome features of the probiotic strains Bifidobacterium longum 379, Bifidobacterium bifidum 1, and Bifidobacterium bifidum 791 and study their antiviral activity.
A. G. Tochilina+9 more
semanticscholar +1 more source
Clinical application of high throughput molecular screening techniques for pharmacogenomics. [PDF]
Genetic analysis is one of the fastest-growing areas of clinical diagnostics. Fortunately, as our knowledge of clinically relevant genetic variants rapidly expands, so does our ability to detect these variants in patient samples.
Schrijver, Iris, Wiita, Arun P
core +1 more source
Effects of keratinocyte-derived and fibroblast-derived exosomes on human epidermal melanocytes.
BACKGROUND Exosomes have been demonstrated to carry proteins, membrane lipids, mRNAs and microRNAs which can be transferred to surrounding cells and regulate the functions of those recipient cells.
Hai-xia Shi+3 more
semanticscholar +1 more source
Review of the initial validation and characterization of a chicken 3K SNP array. [PDF]
In 2004 the chicken genome sequence and more than 2.8 million single nucleotide polymorphisms (SNPs) were reported. This information greatly enhanced the ability of poultry scientists to understand chicken biology, especially with respect to ...
Crooijmans, R.P.M.A.+9 more
core +2 more sources
Aim To present the pathohistological and clinical characteristics of five Croatian families with Alport spectrum disorders caused by splice acceptor pathogenic variant c.193-2A>C in COL4A4 at the genomic position chr2:227985866.
Petar Šenjug+10 more
semanticscholar +1 more source
Transcriptomic profiling of human breast and melanoma cells selected by migration through narrow constraints [PDF]
The metastatic spread of cancer cells is a step-wise process that starts with dissociation from primary tumours and local invasion of adjacent tissues.
Clark, William+4 more
core +1 more source