Results 211 to 220 of about 94,759 (256)

A Simple and Fast Optical Clearing Method for Whole‐Mount Fluorescence In Situ Hybridization (FISH) Imaging

Journal of Biophotonics, EarlyView.
In this article, we present 3D‐LIMPID‐FISH: a simple and fast optical clearing method for acquiring 3D images of fluorescence in situ hybridization probes. Figures and movies are provided to showcase the technology, and a detailed protocol is provided to replicate the result.
Junwoo Suh, Yehe Liu, Jordan Smith, Michiko Watanabe, Andrew M. Rollins, Michael W. Jenkins   +5 more
wiley   +1 more source

Impact of pro-inflammatory cytokine preconditioning on metabolism and extracellular vesicles in feline mesenchymal stromal cells: a preliminary study. [PDF]

Stem Cells
Soltero-Rivera M, Arzi B, Bourebaba L, Marycz K.   +3 more
europepmc   +1 more source

ATAC‐seq in Emerging Model Organisms: Challenges and Strategies

Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, EarlyView.
Principle of ATAC‐seq (left), overview of arthropod species with published ATAC‐seq data (middle), summary of experimental design (right). ABSTRACT The Assay for Transposase‐Accessible Chromatin with sequencing (ATAC‐seq) is a versatile and widely utilized method for identifying potential regulatory regions, such as promoters and enhancers, within a ...
Duğçar Ebrar Erdoğan, Shadi Karimifard, Mozhgan Khodadadi, Liucong Ling, Luisa Linke, Ana Catalán, Vincent Doublet, Amanda Glaser‐Schmitt, Oliver Niehuis, Katja Nowick, Antonella Soro, Natascha Turetzek, Barbara Feldmeyer, Nico Posnien   +13 more
wiley   +1 more source

Protocol for adaptive laboratory evolution of S. cerevisiae by PEG/LiAc transformation and sequencing. [PDF]

STAR Protoc
Jiang B, Xiao C, Liu L.
europepmc   +1 more source

Ecogenomics and limnological dynamics of a new Thiocapsa species blooming in the whole water column of a karstic lake

Limnology and Oceanography, Volume 70, Issue 7, Page 1849-1866, July 2025.
Abstract Purple sulfur bacteria (PSB) of the family Chromatiaceae (Gammaproteobacteria) can perform chemo‐ and photo‐lithoautotrophy (through anoxygenic photosynthesis) in anoxic layers of freshwater stratified (including meromictic) lakes. This group has been extensively studied via physiological and ecological approaches, albeit their genomics has ...
Pedro J. Cabello‐Yeves, Antonio Picazo, Carlos Rochera, Daniel Morant, Eduardo Vicente, David J. Scanlan, Richard J. Puxty, Antonio Camacho   +7 more
wiley   +1 more source

Jurkat T-cell lines exhibit marked genomic instability affecting karyotype, mutational profile, gene expression, immunophenotype and function. [PDF]

Sci Rep
Wilson A, Dasyam N, O'Hagan F, Farrell D, Turner C, Jay A, Schmidt A, Beddow R, Lillis T, Chin T, Perret R, McCulley M, Weinkove R.   +12 more
europepmc   +1 more source

Identification and Biological Characterization of a Novel NRF2 Activator Molecule Released From the Membranes of Heat‐Treated Bifidobacterium breve NCC 2950

Molecular Nutrition &Food Research, EarlyView.
We have shed light on the mode of action behind the postbiotic form of Bifidobacterium breve NCC 2950. Heat treatment of this strain at 90°C leads to the production of 4‐oxo‐2‐pentenoic acid (OPA), which activates NRF2 host response, improving oxidative stress resistance and decreasing inflammation. ABSTRACT Postbiotics are defined as a “preparation of
Francis Foata, Stéphane Duboux, Sébastien Herzig, Federico Sizzano, Jonathan Thevenet, Philippe Guy, Serge Rezzi, Sylviane Métairon, Bertrand Bourqui, Ivan Montoliu, Annick Mercenier, Nabil Bosco   +11 more
wiley   +1 more source

Filamin A editing in myeloid cells reduces intestinal inflammation and protects from colitis. [PDF]

J Exp Med
Gawish R, Varada R, Deckert F, Hladik A, Steinbichl L, Cimatti L, Milanovic K, Jain M, Torgasheva N, Tanzer A, De Paepe K, Van de Wiele T, Hausmann B, Lang M, Pechhacker M, Ibrahim N, De Vries I, Brostjan C, Sixt M, Gasche C, Boon L, Berry D, Jantsch MF, Pereira FC, Vesely C.   +24 more
europepmc   +1 more source

Sample Preparation for Multi‐Omics Analysis: Considerations and Guidance for Identifying the Ideal Workflow

PROTEOMICS, EarlyView.
ABSTRACT Advances in methodologies and technologies over the past decade have led to an unprecedented depth of analysis of a cell's biomolecules, with entire genomes able to be sequenced in hours and up to 10,000 transcripts or ORF products (proteins) able to be quantified from a single cell.
Breyer Woodland, Luke A. Farrell, Lana Brockbals, Maria Rezcallah, Aiden Brennan, Emily J. Sunnucks, Sam T. Gould, Aleksandra M. Stanczak, Matthew B. O'Rourke, Matthew P. Padula   +9 more
wiley   +1 more source

Bacterial translocation and gut microbiome imbalance in an experimental infection model of legionellosis in guinea pigs. [PDF]

BMC Microbiol
Cai X, Xu M, Lu Y, Shen W, Kang J, Wang W, Chen Y.   +6 more
europepmc   +1 more source