Results 31 to 40 of about 94,759 (256)

Assembling the perfect bacterial genome using Oxford Nanopore and Illumina sequencing

PLoS Comput. Biol., 2023
A perfect bacterial genome assembly is one where the assembled sequence is an exact match for the organism’s genome—each replicon sequence is complete and contains no errors. While this has been difficult to achieve in the past, improvements in long-read
R. Wick, L. Judd, K. Holt
semanticscholar   +1 more source

Nanopore Is Preferable over Illumina for 16S Amplicon Sequencing of the Gut Microbiota When Species-Level Taxonomic Classification, Accurate Estimation of Richness, or Focus on Rare Taxa Is Required

Microorganisms, 2023
Nanopore sequencing is a promising technology used for 16S rRNA gene amplicon sequencing as it can provide full-length 16S reads and has a low up-front cost that allows research groups to set up their own sequencing workflows.
Márton Szoboszlay, Laetitia Schramm, David Pinzauti, J. Scerri, A. Sandionigi, Manuele Biazzo   +5 more
semanticscholar   +1 more source

A murine herpesvirus closely related to ubiquitous human herpesviruses causes T-cell depletion [PDF]

, 2017
The human roseoloviruses human herpesvirus 6A (HHV-6A), HHV-6B, and HHV-7 comprise the Roseolovirus genus of the human Betaherpesvirinae subfamily. Infections with these viruses have been implicated in many
Beatrice Plougastel-Douglas, Daved H. Fremont, David Wang, Elvin J. Lauron, Eugene Park, Guoyan Zhao, Ian B. Harvey, Jennifer Poursine-Laurent, Swapneel J. Patel, Vinay R. Penna, Wandy L. Beatty, Wayne M. Yokoyama   +11 more
core   +2 more sources

Comparison of Oxford Nanopore Technologies and Illumina MiSeq sequencing with mock communities and agricultural soil

Scientific Reports, 2023
Illumina MiSeq is the current standard for characterizing microbial communities in soil. The newer alternative, Oxford Nanopore Technologies MinION sequencer, is quickly gaining popularity because of the low initial cost and longer sequence reads ...
B. Stevens, Tim B. Creed, C. L. Reardon, D. Manter   +3 more
semanticscholar   +1 more source

Merging DNA metabarcoding and ecological network analysis to understand and build resilient terrestrial ecosystems [PDF]

, 2016
Summary 1. Significant advances in both mathematical and molecular approaches in ecology offer unprecedented opportunities to describe and understand ecosystem functioning.
Evans, Darren M., Kitson, James J. N., Kitson, James J.N., Lunt, David H., Pocock, Michael J. O., Pocock, Michael J.O., Straw, Nigel A.   +6 more
core   +2 more sources

Mapping translocation breakpoints by next-generation sequencing.

Genome Research, 2008
Balanced chromosome rearrangements (BCRs) can cause genetic diseases by disrupting or inactivating specific genes, and the characterization of breakpoints in disease-associated BCRs has been instrumental in the molecular elucidation of a wide variety of ...
Wei Chen, V. Kalscheuer, A. Tzschach, C. Menzel, R. Ullmann, Marcel H. Schulz, F. Erdogan, Na Li, Z. Kijas, G. Arkesteijn, I. L. Pajares, Margret Goetz-Sothmann, U. Heinrich, I. Rost, A. Dufke, U. Grasshoff, B. Glaeser, M. Vingron, H. Ropers   +18 more
semanticscholar   +1 more source

A comparison of five Illumina, Ion Torrent, and nanopore sequencing technology-based approaches for whole genome sequencing of SARS-CoV-2

European Journal of Clinical Microbiology and Infectious Diseases, 2023
Rapid identification of the rise and spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern remains critical for monitoring of the efficacy of diagnostics, therapeutics, vaccines, and control strategies.
E. Carbo, K. Mourik, S. A. Boers, B. O. Munnink, D. Nieuwenhuijse, M. Jonges, M. Welkers, S. Matamoros, J. van Harinxma thoe Slooten, M. Kraakman, Evita Karelioti, David L. M. van der Meer, K. Veldkamp, A. Kroes, I. Sidorov, J. D. de Vries   +15 more
semanticscholar   +1 more source

The chloride channel cystic fibrosis transmembrane conductance regulator (CFTR) controls cellular quiescence by hyperpolarizing the cell membrane during diapause in the crustacean Artemia [PDF]

, 2019
Cellular quiescence, a reversible state in which growth, proliferation, and other cellular activities are arrested, is important for self-renewal, differentiation, development, regeneration, and stress resistance.
Bossier, Peter, De Vos, Stephanie, Jia, Wen-Huan, Jin, Feng, Li, An-Qi, Liu, Xu, Sun, Zhan-Peng, Van Stappen, Gilbert, Yang, Jin-Shu, Yang, Wei-Jun, Zhu, Lin   +10 more
core   +1 more source

Shotgun sequencing to determine corneal infection

American Journal of Ophthalmology Case Reports, 2020
Purpose: To investigate if shotgun-sequencing method could be useful in detailed diagnosis of herpes simplex virus (HSV) infection and compare it with the conventional diagnostic method.
Mohit Parekh, Vito Romano, Antonella Franch, Pia Leon, Federica Birattari, Davide Borroni, Stephen B. Kaye, Diego Ponzin, Sajjad Ahmad, Stefano Ferrari   +9 more
doaj  

The well-coordinated linkage between acidogenicity and aciduricity via insoluble glucans on the surface of Streptococcus mutans. [PDF]

, 2015
Streptococcus mutans is considered the principal cariogenic bacterium for dental caries. Despite the recognition of their importance for cariogenesis, the possible coordination among S.
Guo, Lihong, He, Xuesong, Lux, Renate, McLean, Jeffrey S, Shi, Wenyuan   +4 more
core   +1 more source