We provide a step‐by‐step guide for producing E140 antigen fragments from Plasmodium berghei (Pb1) and Plasmodium vivax (Pv1). Pb1/Pv1 are expressed in E. coli, solubilized by freeze–thawing, refolded by slow dilution, purified by affinity chromatography (IMAC), then concentrated and subjected to quality control.
Rodolfo Ferreira Marques+5 more
wiley +1 more source
Body image flexibility and embodiment in eating disorders: a mixed-methods approach combining network analysis and pilot exposure protocol. [PDF]
Meneguzzo P+5 more
europepmc +1 more source
Tri-band vehicle and vessel dataset for artificial intelligence research. [PDF]
Liu Y+9 more
europepmc +1 more source
A multi-filter deep transfer learning framework for image-based autism spectrum disorder detection. [PDF]
Contreras RC+5 more
europepmc +1 more source
Dimensional Accuracy Evaluation of Single-Layer Prints in Direct Ink Writing Based on Machine Vision. [PDF]
Tu Y+8 more
europepmc +1 more source
Novel mAbs could detect cancer‐associated membrane proteins on various type of pancreatic ductal adenocarcinomas (PDAC) by flow cytometry with viable PDAC cells and by immunohistochemistry with PDAC tissues. Results of protein expression were substantiated by mRNA expression by The Cancer Genome Atlas.
Takashi Nakano+16 more
wiley +1 more source
Correction: The utility of customised tissue probability maps and templates for patients with idiopathic normal pressure hydrocephalus: a computational anatomy toolbox (CAT12) study. [PDF]
Kanno S+19 more
europepmc +1 more source
HDAC4 regulates apoptosis in Acan‐CreERT2;HDAC4d/d mice with osteoarthritis by downregulating ATF4
Using the AcanCreERT2;HDAC4fl/fl genetic mouse model, we further investigated the role of histone deacetylase 4 (HDAC4) in chondrocyte apoptosis. We found that knocking down HDAC4 may be associated with chondrocyte apoptosis by upregulating the activating transcription factor 4 (ATF4)/CAAT/enhancer binding protein homologous (CHOP) signaling pathway ...
Jingrui Huang+11 more
wiley +1 more source
Natural averaging may complement known biological constraints in sexual reproduction's advantages over asexual in conserving species quantitative traits. [PDF]
Marron A, Szekely S, Cohen IR, Harel D.
europepmc +1 more source