Results 81 to 90 of about 12,292,225 (304)

Spatial biology in cancer epigenetics

Molecular Oncology, EarlyView.
Spatial epigenomics combines molecular profiling with tissue architecture to reveal how gene regulation is organized within intact tissues. In cancer, these technologies uncover the mechanisms driving tumor heterogeneity and microenvironmental interactions, opening new opportunities for biomarker discovery and precision medicine.
Eva Crespo‐García, Manel Esteller
wiley   +1 more source

On image reconstruction from multiscale top points

, 2005
Image reconstruction from a fiducial collection of scale space interest points and attributes (e.g. in terms of image derivatives) can be used to make the amount of information contained in them explicit.
Kanters, Frans, Lillholm, Martin, Platel, Bram, Duits, Remco, Kimmel, R., Florack, Luc, Romeny, Bart M. ter Haar, Janssen, Bart J., Sochen, N.A., Weickert, J.   +9 more
core   +1 more source

Intrapatient tumour heterogeneity and clonal evolution in an autopsy study of metastatic salivary gland cancer

Molecular Oncology, EarlyView.
Tumour heterogeneity and clonal evolution of metastatic salivary gland cancer were evaluated in two patients with adenoid carcinoma and one patient with myoepithelial carcinoma. Radiology‐guided autopsy enabled multi‐region sampling (total samples n = 149), followed by whole‐genome sequencing and phylogenetic reconstruction (17 tumour samples, 4–7 per ...
Gerben Lassche, Niels J. van Ruitenbeek, Charlotte Adang, Luke O'Gorman, Pui Yuen Lee, Willemijn M. Klein, Adriana C. H. van Engen‐van Grunsven, Jack A. Schalken, Sander Bervoets, Gerald W. Verhaegh, Carla M. L. van Herpen   +10 more
wiley   +1 more source

Methodology for reconstructing early zebrafish development from in vivo multiphoton microscopy [PDF]

, 2012
Investigating cell dynamics during early zebrafish embryogenesis requires specific image acquisition and analysis strategies. Multiharmonic microscopy, i.e., second- and third-harmonic generations, allows imaging cell divisions and cell membranes in ...
A. Santos, T. Savy, N. Olivier, Luengo-Oroz, Miguel Angel, M. Ledesma-Carbayo, Bourgine, Paul, Olivier, Nicolas, D. Debarre, Savy, Thierry, Debarre, Delphine, Rubio-Guivernau, José L., P. Bourgine, Santos Lleo, Andres de, Faure, Emmanuel, Luengo Oroz, Miguel Ángel, Ledesma Carbayo, María Jesús, M. A. Luengo-Oroz, Pastor Escuredo, David, E. Faure, Duloquin, Louise, J. L. Rubio-Guivernau, N. Peyrieras, Ledesma-Carbayo, María Jesús, Santos, Andrés, E. Beaurepaire, Peyrieras, Nadine, Pastor-Escuredo, David, D. Pastor, L. Duloquin, Rubio Guivernau, José Luis, Beaurepaire, Emmanuel   +30 more
core   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Selecting surface features for accurate multi-camera surface reconstruction [PDF]

, 2009
This paper proposes a novel feature detector for selecting local textures that are suitable for accurate multi-camera surface reconstruction, and in particular planar patch fitting techniques.
Roland Wilson, Wilson, Roland, Thomas Popham, Popham, T. J.   +3 more
core   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Efficient non-linear 3D electrical tomography reconstruction

, 2001
Non-linear electrical tomography imaging can be performed efficiently if certain optimisations are applied to the computational reconstruction process. We present a 3D non-linear reconstruction algorithm based on a regularized conjugate gradient solver ...
Daniell, G.J., Molinari, M., Cox, S.J., Blott, B.H.   +3 more
core   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source