Results 71 to 80 of about 387,829 (316)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Comparative Evaluation of Booster Vaccine Efficacy by Intracoelomic Injection and Immersion with a Whole-Cell Killed Vaccine against Lactococcus petauri Infection in Rainbow Trout (Oncorhynchus mykiss)

Pathogens, 2023
Lactococcus petauri is an important emergent bacterial pathogen of salmonids in the USA. The purpose of this study was to evaluate the protection conferred to rainbow trout (Oncorhynchus mykiss) against L. petauri by formalin-killed vaccines in immersion
Tryssa de Ruyter, Eric Littman, Zeinab Yazdi, Mark Adkison, Alvin Camus, Susan Yun, Timothy J. Welch, William R. Keleher, Esteban Soto   +8 more
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Current possibilities and challenges of using metaverse-like environments and technologies in education

Frontiers in Virtual Reality
This paper presents findings from a stakeholder mapping exercise exploring how higher education professionals perceive and engage with metaverse‐like environments and technologies.
Ágnes Karolina Bakk, Judit Bényei, Paul Ballack, Fernanda Parente   +3 more
doaj   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Natural Fibrous Materials as Eco-Friendly Air Filters

Journal of Natural Fibers
Exposure to particulate matter has various health consequences, and high-efficiency particulate air (HEPA) filters and facial masks are used to remove such pollutants.
Seokhyun Chin, Woori Choi, Sunghwan Jung
doaj   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Clinical rationale for dietary lutein supplementation in long COVID and mRNA vaccine injury syndromes [version 3; peer review: 2 approved]

F1000Research
Lutein, a plant-derived xanthophyl-carotenoid, is an exceptional antioxidant and anti-inflammatory constituent found in food. High dietary intake of lutein is beneficial against eye disease, improves cardiometabolic health, protects from ...
Stephanie Seneff, Anthony M Kyriakopoulos, Greg Nigh, Peter A McCullough   +3 more
doaj   +1 more source

How to Describe and Measure Obstacles of Narrative Immersion in a Film?

Nordicom Review, 2016
The aim of this study is to describe and measure obstacles of narrative immersion in a film. Inspired by a literature review within both game research and film studies, we propose a circular model to describe the dynamic process of different levels of ...
Bjørner Thomas, Magnusson Andreas, Nielsen Robin Pascal   +2 more
doaj   +1 more source

Effect of water immersion on the interlaminar and flexural performance of low cost liquid resin infused carbon fabric composites [PDF]

, 2012
This study investigates some potential benefits of using non-epoxy matrices in carbon fibre composites, targeting specific marine and wind energy applications.
Rebecca Lees, Giuseppe Dell’Anno, Lees, Rebecca, Dell'Anno, Giuseppe   +3 more
core   +1 more source