Photochemical synthesis of a “cage” compound in a microreactor: Rigorous comparison with a batch photoreactor [PDF]
An intramolecular [2 + 2] photocycloaddition is performed in a microphotoreactor (0.81 mL) built by winding FEP tubing around a commercially available Pyrex immersion well in which a medium pressure mercury lamp is inserted.
Aillet, Tristan +3 more
core +3 more sources
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
What Do Cattle Prefer in a Tropical Climate: Water Immersion or Artificial Shade? [PDF]
Animal performance is affected by high air temperature and it is known that shade reduces the absorption of radiant temperature, and water for immersion facilitates heat loss.
Geraldo, A.C.P.M. +3 more
core
Implicit-explicit time integration for the immersed wave equation
Immersed boundary methods simplify mesh generation by embedding the domain of interest into an extended domain that is easy to mesh, introducing the challenge of dealing with cells that intersect the domain boundary. Combined with explicit time integration schemes, the finite cell method introduces a lower bound for the critical time step size ...
Christian Faßbender +4 more
openaire +2 more sources
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
Effects of operating factors on osmotic dehydration of broccoli stalk slices
In this study, effects of sucrose concentration (40–60% w/v), immersion time (1–5 h), and temperature (25–65°C) on water loss (WL) and solute gain (SG) during osmotic dehydration of broccoli stalk slices were quantitatively investigated using response ...
Nora Salina Md Salim +2 more
doaj +1 more source
Carbon fiber reinforced plastics (CFRPs) are widely used as components of marine structures. Thus, it is important to understand the degradation of the mechanical properties and its mechanism under seawater immersion.
Shuhei KOSHIMA +3 more
doaj +1 more source
Researching collaborative artistic practice. [PDF]
In this paper we offer discussion of collaboration in artistic practice, based on a two-and-a-half-year-long research project undertaken by artists/researchers at the University of Waikato, working in collaboration with local performers.
Barbour, Karen +3 more
core +1 more source
The Effects of Different Immersion Method and TIME on the Physico-chemical Characteristics of Skipjack Tuna (Katsuwonus Pelamis) Fish Meal [PDF]
High fat content in a dark fleshed fish can affect on nutritional value because it can lead to odors in fish meal products. Effort Immersion (deffating) is one way to reduce fat content in fish meal products in order to reduce the products from odor ...
Litaay, C. (Christina) +1 more
core +3 more sources

