Results 361 to 370 of about 2,310,619 (425)
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Enzyme immobilization on fibrin
Biotechnology and Bioengineering, 1976The following conclusions can be drawn concerning the utilization of fibrin to immobilized enzyme systems. Fibrin can be used both as a powder or membrane, to covalently immobilize trypsin with retention of activity. Carbon-14 labeled trypsin can be used to estimate the amount of immobilized enzyme on a proteinaceous support.
C. W. R. Wade +2 more
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Enzyme immobilization on tritylagarose
Biotechnology and Bioengineering, 1982AbstractA method is described for the immobilization on tritylated agarose or Sepharose columns of a wide spectrum of enzymes, including types useful in contemporary biochemistry/molecular biology, many of which have never before been reported as immobilized.
Victor Lentini +5 more
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Journal of materials chemistry. B, 2015
A facile approach for the synthesis of enzyme-inorganic hybrid nanoflowers and their application as an immobilized α-chymotrypsin (ChT) reactor (IMER) for highly efficient protein digestion was described.
Yuqing Yin +5 more
semanticscholar +1 more source
A facile approach for the synthesis of enzyme-inorganic hybrid nanoflowers and their application as an immobilized α-chymotrypsin (ChT) reactor (IMER) for highly efficient protein digestion was described.
Yuqing Yin +5 more
semanticscholar +1 more source
Immobilized enzymes: understanding enzyme - surface interactions at the molecular level.
Organic and biomolecular chemistry, 2017Enzymes immobilized on solid supports have important and industrial and medical applications. However, their uses are limited by the significant reductions in activity and stability that often accompany the immobilization process.
M. Hoarau, S. Badieyan, E. Marsh
semanticscholar +1 more source
Accelerating trypsin digestion: the immobilized enzyme reactor.
Bioanalysis, 2014Sample preparation has lagged far behind the evolution of instrumentation used in mass-linked protein analysis. Trypsin digestion, for example, still takes a day, as it did 50 years ago, while mass spectral analyses are achieved in seconds.
F. Regnier, Jinhee Kim
semanticscholar +1 more source
Pellicular immobilized enzymes
Biochimica et Biophysica Acta (BBA) - Enzymology, 1974Abstract Methods for the preparation of shell-structured immobilized enzymes supported by a fluid impervious spherical core are outlined. The preparation of such trypsin resins by coreticulation of the enzyme with a copolymer of maleic anhydride and vinyl methylester in situ on the surface of glass beads is described and some properties of the ...
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Flow-through immobilized enzyme reactors based on monoliths: II. Kinetics study and application.
Journal of Separation Science, 2013In the last decade, the application of monolithic materials has rapidly expanded to the realization of flow-through bioconversion processes. Up to these days, different classes of enzymes such as hydrolases, lyases, and oxidoreductases have been ...
E. Vlakh, T. Tennikova
semanticscholar +1 more source
Advances in immobilized enzyme microbioreactors in capillary electrophoresis.
In Analysis, 2013Miniaturized bioanalytical systems are increasingly being used in the field of biochemical research. Immobilized enzyme microbioreactors in capillary electrophoresis (CE) have been constructed and used to fulfill the increasing demands for miniaturized ...
J. Iqbal, Shoaib Iqbal, C. Müller
semanticscholar +1 more source
Enzyme immobilization in porous solid supports—penetration of immobilized enzyme
Biotechnology and Bioengineering, 1984AbstractThe process of enzyme immobilization under the diffusion‐controlled regime (i.e., fast attachment of enzyme compared to its diffusion) is modeled and theoretically solved in this article. Simple and compact solutions for the penetration depth of immobilized enzyme and the bulk enzyme concentration versus time are presented.
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