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Nanoporous Gold for Enzyme Immobilization
2010Nanoporous gold (NPG) is a material of emerging interest for immobilization of biomolecules, especially enzymes. The material provides a high surface area form of gold that is suitable for physisorption or for covalent modification by self-assembled monolayers.
Keith J. Stine +2 more
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[53] Reactivation of immobilized enzymes
1987Publisher Summary This chapter discusses a common feature of the irreversible inactivation processes that the denatured protein is unable to return to the native state N––that is, to reactivate spontaneously after the removal of the denaturant. The principal mechanism for the inactivation of immobilized enzymes is denaturation due to irreversible ...
Vadim V. Mozhaev +2 more
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Immobilization of enzymes on PTFE surfaces
Biotechnology and Bioengineering, 2001Membranes and powders prepared from PTFE (polytetrafluorethylene) were investigated for their potential use as multifunctional supports for enzymes. The obtained bioactive materials are valuable for the construction of biosensors and enzyme reactors. To allow covalent coupling of enzymes to PTFE, the surface of the material was treated with elementary ...
Michael Keusgen +3 more
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Enzyme immobilized nanomaterials as electrochemical biosensors for detection of biomolecules.
Enzyme and Microbial Technology, 2022Meena Nemiwal +2 more
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[49] Immobilized-enzyme reactors
1976Publisher Summary The development the methods for the immobilization has produced novel forms of biochemical catalyst, which can be used in reactors to carry out conversions of industrial interest. Many different types of reactor have been employed with immobilized enzymes.
P Dunnill, M D Lilly
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Nanodevices for the immobilization of therapeutic enzymes
Critical Reviews in Biotechnology, 2015Therapeutic enzymes are one of the most promising applications of this century in the field of pharmaceutics. Biocatalyst properties can be improved by enzyme immobilization on nano-objects, thereby increasing stability and reusability and also enhancing the targeting to specific tissues and cells.
Bosio, Valeria Elizabeth +4 more
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Enzymes Immobilized on Lumen [PDF]
, 2015 This particular kind of immobilization is generally referred to enzymes immobilized into the membrane lumen surface of tubular membranes for the production of biocatalytic membrane and biocatalytic membrane reactors. A scheme of a cross section of a biocatalytic asymmetric hollow fiber membrane is reported in Fig.
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Current Nanoscience, 2019
Engineering of biocatalysts with the help of immobilization techniques is a worthy approach for the advancement of enzyme function and stability and is finer to the other chemical as well as biological methods. These biocatalysts encapsulation methods actually use very gentle method conditions that hardly affect biocatalysts internal specific ...
Abha Mishra +3 more
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Engineering of biocatalysts with the help of immobilization techniques is a worthy approach for the advancement of enzyme function and stability and is finer to the other chemical as well as biological methods. These biocatalysts encapsulation methods actually use very gentle method conditions that hardly affect biocatalysts internal specific ...
Abha Mishra +3 more
openaire +1 more source
Enzyme Immobilization by Adsorption
2003Much has already been written on the subject of biocatalyst (cell/enzyme) immobilization, and it is not the function of a text such as this to go into all the various aspects of the field. For general reviews on the area, the reader is referred to refs. 1-3.
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Enzyme Immobilization by Entrapment
2003Entrapment methods of immobilization are mostly used in cell immobilization procedures, but have found some application with enzymes, particularly when the enzyme is essentially a dead cell or crude homogenate(1). In theory the entrapped enzyme is not attached to the polymer; its free diffusion is merely restrained. In practice, however, some or all of
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