Results 1 to 10 of about 16,007 (160)

Single Radial Immunodiffusion (SRID) Assay for Quantitative Determination of Recombinant SOD Protein in a Brucellosis Vaccine Candidate: Method Development and Validation [PDF]

Methods and Protocols
Recombinant superoxide dismutase (SOD) protein is a promising antigen candidate for brucellosis vaccines, and its quantitative determination is essential for vaccine quality control, standardization, and regulatory compliance.
Gulnur Nakhanova, Olga Chervyakova, Kamshat Shorayeva, Aigerim Zhakypbek, Sabina Moldagulova, Aknur Ulankyzy, Alisher Omurtay, Yeraly Shayakhmetov, Temirlan Baiseit, Zharkinay Absatova, Aisha Issabek, Gaukhar Shynybekova, Sandugash Sadikaliyeva, Aziz Nakhanov, Kuanysh Jekebekov, Ainar Kossylganova, Karlygash Zhaparkulova, Assem Kalykova, Tolkyn Bekezhanova, Albina Atakanova, Zakir Yershebulov, Kuandyk Zhugunissov   +21 more
doaj   +2 more sources

Comparative assessment of immunodiffusion and enzyme-linked immunosorbent assay used for bovine leukosis diagnosis

Ветеринария сегодня, 2023
The implementation of animal health improvement and disease prevention activities with respect to bovine leukosis involves a need for timely detection of cattle infected with bovine leukaemia virus (BLV) on livestock farms.
A. R. Mustafayev, M. O. Baratov
doaj   +1 more source

Diagnostic Methods and Risk Factors for Severe Disease and Mortality in Blastomycosis: A Retrospective Cohort Study

Journal of Fungi, 2021
Background: Blastomycosis can cause severe disease with progressive respiratory failure and dissemination even in immunocompetent individuals. We sought to evaluate risk factors for severe disease and mortality using clinical and laboratory data within a
Timothy R. O’Dowd, Jack W. Mc Hugh, Elitza S. Theel, Nancy L. Wengenack, John C. O’Horo, Mark J. Enzler, Paschalis Vergidis   +6 more
doaj   +1 more source

Antibody Titer Prediction from Serum Immunodiffusion Test of Patients with Paracoccidioidomycosis Using Infrared Spectroscopy and Chemometrics

Analytica, 2023
Paracoccidioidomycosis (PCM) is a systemic mycosis caused by fungi of the genus Paracoccidioides. Serological tests are auxiliary in the diagnosis of PCM.
Alessandra Koehler, Maria Lúcia Scroferneker, Paulo Cezar de Moraes, Beatriz Aparecida Soares Pereira, Ricardo de Souza Cavalcante, Rinaldo Pôncio Mendes, Valeriano Antonio Corbellini   +6 more
doaj   +1 more source

A Laboratory Exercise Simulating Antibody and Antigen Reactions of the Ouchterlony Double Immunodiffusion Assay Using Inorganic Salts

Journal of Microbiology & Biology Education, 2021
The Ouchterlony double immunodiffusion assay is a serological technique used in the detection of antibodies and antigens for diagnostic purposes and also used in immunology laboratory courses as a common teaching assay where students observe the ...
Mustafa G. Mujtaba, Tara Baliban, Jamini Bhagu, Michael Herrera   +3 more
doaj   +1 more source

Immunodiffusion assay as a method of bovine leucosis post-mortem diagnosis

Ветеринария сегодня, 2022
New method of post-mortem diagnosis of bovine leukosis is proposed and it involves use of agar gel immunodiffusion assay. The proposed method allows for the detection of antibodies against bovine leukemia virus (BLV) antigen located in the muscle and ...
A. R. Mustafayev
doaj   +1 more source

Development, certification, and use of a pharmacopoeial standard for the content of immunoglobulin A in human immunoglobulins for parenteral administration

Биопрепараты: Профилактика, диагностика, лечение, 2023
Scientific relevance. The immunoglobulin A (IgA) impurity content in parenteral human immunoglobulins should be determined in accordance with the State Pharmacopoeia of the Russian Federation by kinetic nephelometry, radial immunodiffusion, or enzyme ...
A. V. Nechaev, E. Yu. Kudasheva, E. L. Postnova, R. A. Volkova, O. V. Fadeikina, I. V. Borisevich, A. A. Movsesyants   +6 more
doaj   +1 more source

Development of approaches to the diagnosis of cattle leukemia in the system of antiepizootic measures in the Belgorod Region

BIO Web of Conferences, 2021
The aim of the research is the use of serological and molecular genetic methods for detecting virus-infected cattle leukemia, as well as determining the significance of PCR in identifying BLV infected calves in the system of antiepizootic health measures.
Kovalenko Anatoly, Donnik Irina, Dorofeev Andrey, Belyaeva Svetlana, Yavnikov Nazar, Oskolskaya Victoria, Karaychentsev Danila, Petropavlovskiy Maxim   +7 more
doaj   +1 more source

Whole-cell antigenicity data support sequence-based kinetoplastid taxonomy

Memorias do Instituto Oswaldo Cruz, 2013
Early immunological data, obtained by immunodiffusion and immunoelectrophoresis, on the whole-cell antigenicity of kinetoplastid protozoa were retrieved and used to construct a dendrogram of antigenic distances.
Bernard Couvreur
doaj   +1 more source

Alpha Toxin Purification and Antibody Production Against Local Strain of Clostridium septicum NH2 [PDF]

Iranian Journal of Veterinary Medicine, 2019
BACKGROUND: Clostridium septicum has played a significant role as a causative agent of many acute fetal diseases in man and animals. Alpha- toxin is the main factor in the pathogenesis of C.
Mohsen Najafi Najafi, Mohammad Hemmaty, Khadijeh Moridi   +2 more
doaj   +1 more source