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Quantitative Radial Immunodiffusion
1979Mancini et al. (1965) developed a very simple method to quantitate antigens using gel diffusion. The principle is this: The antigen is placed in a well punched into an agar layer. The agar was mixed while melted at 45°-50°C with the corresponding antibody.
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1996
Single radial immunodiffusion is used extensively for the quantitative estimation of antigens (1). In this method, the antigen--antibody precipitation is made more sensitive than in double immunodiffusion (see Chapter 135) by the incorporation of the antiserum in the agar solution before the gel is made (2). Thus, the antiserum is uniformly distributed
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Single radial immunodiffusion is used extensively for the quantitative estimation of antigens (1). In this method, the antigen--antibody precipitation is made more sensitive than in double immunodiffusion (see Chapter 135) by the incorporation of the antiserum in the agar solution before the gel is made (2). Thus, the antiserum is uniformly distributed
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Immunodiffusion and Immunoelectrophoretic Techniques
Vox Sanguinis, 1970F, Pesendorfer +2 more
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CHROMATOGRAPHY AND IMMUNODIFFUSION
The Lancet, 1967A. Agostoni, C. Vergani, B. Lomanto
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Detecting Microalbuminuria by Radial Immunodiffusion
Diabetic Medicine, 1985D, Cheţa, G, Stoica
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INTERPRETATION OF IMMUNODIFFUSION TESTS
Annual Review of Microbiology, 1960openaire +2 more sources