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A simplified immunoenzyme antigen binding technique as an approach for immunodiagnosis
Journal of Immunological Methods, 1980A simple immunoenzyme technique is described in which the enzyme beta-galactosidase of E. coli (Z) is used as a marker. The principle of the test is based on the ability of the cells to bind the antigen. Conditions of the assay using Z both as the antigen and marker are described.
Bahr, G, Modabber, F Z
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Research in Veterinary Science, 1983
Several antigens including immunoglobulin light and heavy chains, C3, macrophage enzymes and various brain proteins were demonstrated immunohistologically in paraffin sections of canine tissues. The indirect immunoperoxidase, the unlabelled antibody enzyme (PAP), double bridging PAP, and biotinavidin-peroxidase (BAP) methods were compared.
M, Vandevelde +4 more
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Several antigens including immunoglobulin light and heavy chains, C3, macrophage enzymes and various brain proteins were demonstrated immunohistologically in paraffin sections of canine tissues. The indirect immunoperoxidase, the unlabelled antibody enzyme (PAP), double bridging PAP, and biotinavidin-peroxidase (BAP) methods were compared.
M, Vandevelde +4 more
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Bloodtyping in Human Dental Pulp by Immunoenzyme Techniques
1988Genetically determined markers increase the chances of individual identification of human cadavers, especially in cases in which tests cannot be made by direct methods because of detoriation, in putrefied and skeletonized cadavers.
L. Pötsch-Schneider, L. Penzes
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Localization of cathepsin L in rat kidney revealed by immunoenzyme and immunogold techniques
Histochemistry, 1988Localization of cathepsin L in rat kidney was investigated by immunocytochemical techniques. Kidneys were fixed by perfusion and embedded in Epon or Lowicryl K4M without postosmication. For light microscopy (LM), semi-thin sections of the Epon-embedded material were stained by the immunoenzyme technique after removal of epoxy resin.
S, Yokota, Y, Nishimura, K, Kato
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Use of immunoenzyme technique for the detection of Aujeszky's disease virus in cell culture
Veterinary Record, 1978 J, Roszkowski +3 more
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Analytical Biochemistry, 1973
Abstract Conditions have been established for estimating antibody potency by conducting an antigen-antibody reaction whose product is soluble and where the antigen is an enzyme whose activity is reduced on combination with antibody. The antigen preparations were made from placental alkaline phosphatase, and antisera from rabbits were collected.
W H, Fishman +4 more
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Abstract Conditions have been established for estimating antibody potency by conducting an antigen-antibody reaction whose product is soluble and where the antigen is an enzyme whose activity is reduced on combination with antibody. The antigen preparations were made from placental alkaline phosphatase, and antisera from rabbits were collected.
W H, Fishman +4 more
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[48] Immunoenzymic techniques for biomedical analysis
1976Publisher Summary This chapter discusses the immunoenzymic techniques for biomedical analysis. Since the first publications on the preparation and uses of enzyme labeled proteins appeared, there has been continuous development in this area. An appreciable number of immunoenzymic techniques, which make use of enzymes coupled to antigens or antibodies,
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