Results 171 to 180 of about 9,412,541 (217)
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Journal of Immunological Methods, 1989
Simultaneous detection of multiple tissue epitopes with an overlapping distribution pattern by monoclonal antibodies is sometimes needed for routine immunohistological evaluations. Therefore, multistep double and triple immunoenzymatic methods using antibodies from the same species or Ig (sub)class have been developed. Since only commercially available
Hendrik Jan Houthoff +3 more
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Simultaneous detection of multiple tissue epitopes with an overlapping distribution pattern by monoclonal antibodies is sometimes needed for routine immunohistological evaluations. Therefore, multistep double and triple immunoenzymatic methods using antibodies from the same species or Ig (sub)class have been developed. Since only commercially available
Hendrik Jan Houthoff +3 more
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Publisher Summary This chapter focuses on immunofluorescence techniques. The primary disadvantage is that fresh tissue is required, fixed paraffin-embedded tissue being generally unsuitable. The primary antibody is applied to the section in high excess followed by labeled primary antigen.
C.F.A. Culling, R.T. Allison, W.T. Barr
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A simplified immunoenzyme antigen binding technique as an approach for immunodiagnosis
A simple immunoenzyme technique is described in which the enzyme beta-galactosidase of E. coli (Z) is used as a marker. The principle of the test is based on the ability of the cells to bind the antigen. Conditions of the assay using Z both as the antigen and marker are described.
George Bahr +3 more
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Human pituitary glands and pituitary adenomas, obtained by surgery or at autopsy, have been stained with lead hematoxylin and the results were compared with those of other histochemical procedures, including the immunoperoxidase technique. Lead hematoxylin positivity was found in the cytoplasm of melanocorticotroph, thyrotroph as well as gonadotroph ...
Kalman Kovacs, Calvin Ezrin, Nancy Ryan
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SUMMARY.The present report describes a simple, reproducible, semi‐quantitative immunohistochemical assay for the detection of antiplatelet antibody. Evaluation of the technique in 10 children with active idiopathic (autoimmune) thrombocytopenic purpura (ITP), seven children with thrombocytopenia due to other causes, and 12 normal individuals revealed ...
William Krivit +4 more
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Peripheral nerve biopsies from patients with leprosy were stained with anti-Mycobacterium bovis (BCG) in a peroxidase-antiperoxidase (PAP) system to demonstrate intraneural mycobacterial antigens. Most M. leprae antigens have been shown to cross-react with BCG.
Ayele Belehu +3 more
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Use of immunoenzyme technique for the detection of Aujeszky's disease virus in cell culture
M Bartoszcze +3 more
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Immunoenzyme Techniques in Dermatopathology
International Journal of Dermatology, 1986G. Schaumburg‐Lever
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According to the competitive ELISA test data, new preparations of monoclonal antibodies (MAb) to respiratory syncytial virus (RSV) differed in their blocking activity and they were directed to 3 different virus epitopes. MAb 9C5 and MAb 131-2A (CDC, Atlanta) competed against each other strongly and they were directed to epitope F1a of RSV F-protein ...
A A, Sominina +9 more
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