Results 171 to 180 of about 3,048 (216)

Immunochemical studies of an immunoglobulin M-immunoglobulin G mixed cryoglobulin

Clinical Immunology and Immunopathology, 1978
Abstract A mixed IgG-IgM cryoglobulin was isolated from a patient with arthritis and glomerulonephritis. Electron microscopic examination of both cryoprecipitate and glomerular deposits revealed unusual structures designated as “cylindrical and annular bodies.” The IgM component was monoclonal and had antibody activity against IgG molecules ...
F, Chenais, H H, Fudenberg, A C, Wang
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Isolation of Murine and Human Immunoglobulin M and Murine Immunoglobulin D

Current Protocols in Immunology, 2009
AbstractThis unit describes two classical protocols for the purification of IgM—dialysis of ascites fluid, tissue culture medium, or bioreactor supernatants against distilled water to precipitate pure IgM, and ammonium sulfate precipitation. Both protocols can be followed by size‐exclusion chromatography to obtain a highly purified product.
Sarah M, Andrew, Julie A, Titus, Ashok, Amin, Richard, Coico   +3 more
openaire   +2 more sources

Immunoglobulin-M purification — Challenges and perspectives

Biotechnology Advances, 2011
Extensive research in the past two decades has led to the realization of Immunoglobulin-M (IgM) as a potential therapeutic and diagnostic agent. In order to fully exploit the potential of IgM, large quantities, in a highly pure and active form, must be available at low cost for performing clinical trials, characterization studies and quantitative ...
Satyen, Gautam, Kai-Chee, Loh
openaire   +2 more sources

Selective Immunoglobulin M Deficiency in Foals

Journal of the American Veterinary Medical Association, 1977
SUMMARY Selective immunoglobulin M deficiency was diagnosed in 5 foals, on the basis of reduced serum immunoglobulin M concentrations (more than 2 standard deviations below the normal mean). All 5 foals had clinical signs or lesions involving the respiratory tract.
L E, Perryman, T C, McGuire, B J, Hilbert   +2 more
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Structural studies on bovine immunoglobulin M

Biochimica et Biophysica Acta (BBA) - Protein Structure, 1972
Abstract Heavy (μ) and light polypeptide chains were prepared from normal bovine IgM and were found to have molecular weights of 61 800 and 22 800. Heavy chain from a human pathological IgM had a molecular weight of 65 200. Bovine μ-chain contained 12% carbohydrate but the light chain contained less than 0.5%. The human μ-chain was found to have 15%
D, Beale, N, Buttress
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Conjugation of Immunoglobulin M to Gold Nanoparticles

Current Nanoscience, 2011
Due to unique optical properties, facile bioconjugation, and biocompatibility, colloidal gold nanoparticles have been widely used for nuclear targeting, gene and drug delivery, and biomedical diagnosis and therapeutics. Various peptides, proteins, and polymers, etc. have been utilized to functionalize gold nanoparticles. Especially antibodies-AuNPs
Wang, Hui, Dimitrov, Krassen
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Immunoglobulin M in murray valley encephalitis

Pathology, 1975
Twelve clinical cases of Murray Valley encephalitis are described, in which the sero-diagnosis was confirmed by the detection of Murray Valley encephalitis immunoglobulin M.
M A, Wiemers, N D, Stallman
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Neutralization by Immunoglobulin M

1993
The pentameric nature of IgM ensures high avidity (about 107-fold greater than Fab) for structures bearing repeated identical antigenic determinants, but in general IgM is of low specificity since its variable region genes are not subject to hypermutation modification that occurs in cells synthesizing IgG or IgA and increases the affinity of these ...
openaire   +1 more source

Piezoelectric immunosensor for the detection of immunoglobulin M

The Analyst, 1995
A piezoelectric immunosensor has been developed for the determination of human IgM. The crystals are AT-cut and have a basic resonant frequency of 9 MHz. Immobilization of goat antihuman IgM antibodies to the crystals' surfaces was accomplished via a CNBr-activated copolymer coating of 2-hydroxyethyl methacrylate and methylmethacrylate.
X, Chu, Z H, Lin, G L, Shen, R Q, Yu
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Primary Selective Deficiency of Immunoglobulin M

Internal Medicine Journal, 1978
Summary: Selective deficiency of IgM as a primary disorder is seldom encountered1–3, and the syndrome remains ill‐defined. It is generally accepted that for a diagnosis to be made, the serum IgM level should be consistently below 2 SD of normal, no other immunodeficiencies are present, and it is not secondary to other disease processes. This report of
Y H, Thong, G M, Maxwell
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