Results 331 to 340 of about 25,748,471 (370)
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Human hybrid immunoglobulin M containing immunoglobulin A
In Vitro Cellular & Developmental Biology, 1988Some hybridoma clones made by fusion of a human lymphoblastoid cell line, HO323 with human B lymphocytes, secreted not only IgA but also IgM-like immunoglobulin molecules. The IgM-like immunoglobulin had a molecular size of 900 K which corresponded to that of IgM. Immunochemical analyses revealed that the IgM-like immunoglobulin contained two monomeric
H, Shinmoto +4 more
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Pregnancy in immunoglobulin M nephropathy
Journal of Obstetrics and Gynaecology Research, 2017AbstractImmunoglobulin M nephropathy is an uncommon glomerular disease and a relatively less recognized clinico‐immunopathological entity in the domain of glomerulonephritis, often thought to be a bridge between minimal change disease and focal segmental glomerulosclerosis.
Meryem, Hocaoğlu +3 more
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Polymerization of human immunoglobulin M
Biochemistry, 1978The repolymerization of human IgM following mild reductive cleavage was studied as a model for intracellular polymer assembly. Repolymerization was found to require the presence of J chain and a disulfide exchanging system which could be furnished either intrinsically by the use of the monofunctional thiol mercaptoethylamine or extrinsically by the ...
C E, Wilde, M E, Koshland
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Purification of Immunoglobulin
AbstractThis unit describes two classical protocols for the purification of IgM ‐ dialysis of ascites fluid, tissue culture medium, or bioreactor supernatants against distilled water to precipitate pure IgM, and ammonium sulfate precipitation. Both protocols can be followed by size‐exclusion chromatography to obtain a highly purified product.
S M, Andrew +3 more
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European Journal of Immunology, 1980
AbstractMutants of a hybridoma line secreting IgM with anti‐trinitrophenyl specificity were enriched by coupling the hapten to the cell surface and incubating the cells in the presence of complement. In this way, cells secreting wild‐type Ig commit suicide, whereas cells secreting IgM with reduced lytic activity preferentially survive.
Georges Köhler, Marc J. Shulman
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AbstractMutants of a hybridoma line secreting IgM with anti‐trinitrophenyl specificity were enriched by coupling the hapten to the cell surface and incubating the cells in the presence of complement. In this way, cells secreting wild‐type Ig commit suicide, whereas cells secreting IgM with reduced lytic activity preferentially survive.
Georges Köhler, Marc J. Shulman
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Immunoglobulin M as a vaccine adjuvant
Medical Hypotheses, 2011Vaccines are effective in preventing disease by stimulating the immune system and sustaining an immune response towards eradication of pathogens and diseased cells. However, designing successful vaccines is not always straightforward. For a vaccine to be successful, antigen-presenting cells (APC) need to be stimulated, primarily by adjuvants, towards a
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Immunoglobulin G paraprotein cross-reactivity with immunoglobulin M measurement
Annals of Clinical Biochemistry: International Journal of Laboratory Medicine, 2008We report a case of IgG paraprotein cross-reactivity with IgM measurement in a patient with IgGκ multiple myeloma and associated IgA and IgM immune paresis. IgM measurements using a Beckman-Coulter Synchron LX IgM reagent initially gave unmeasurably low IgM concentrations (<0.3 g/L) but IgM quantitation using later batches of reagent gave IgM ...
Robert C, Hawkins, Lian-King, Lee
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Immunochemical studies of an immunoglobulin M-immunoglobulin G mixed cryoglobulin
Clinical Immunology and Immunopathology, 1978Abstract A mixed IgG-IgM cryoglobulin was isolated from a patient with arthritis and glomerulonephritis. Electron microscopic examination of both cryoprecipitate and glomerular deposits revealed unusual structures designated as “cylindrical and annular bodies.” The IgM component was monoclonal and had antibody activity against IgG molecules ...
F, Chenais, H H, Fudenberg, A C, Wang
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Isolation of Murine and Human Immunoglobulin M and Murine Immunoglobulin D
Current Protocols in Immunology, 2009AbstractThis unit describes two classical protocols for the purification of IgM—dialysis of ascites fluid, tissue culture medium, or bioreactor supernatants against distilled water to precipitate pure IgM, and ammonium sulfate precipitation. Both protocols can be followed by size‐exclusion chromatography to obtain a highly purified product.
Sarah M, Andrew +3 more
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Selective Immunoglobulin M Deficiency in Foals
Journal of the American Veterinary Medical Association, 1977SUMMARY Selective immunoglobulin M deficiency was diagnosed in 5 foals, on the basis of reduced serum immunoglobulin M concentrations (more than 2 standard deviations below the normal mean). All 5 foals had clinical signs or lesions involving the respiratory tract.
L E, Perryman +2 more
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